IL-33 Polyclonal antibody

IL-33 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28035-1-AP

Synonyms

9230117N10Rik, Il 33, Il1f11, Il33, IL-33, interleukin 33, NF HEV

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Validation Data Gallery

• 

  WB analysis of mouse lung using 28035-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 28035-1-AP (il33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 28035-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 28035-1-AP (il33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 28035-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 28035-1-AP (il33 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse colon tissue, rat lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28035-1-AP targets IL-33 in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-33 fusion protein Ag27846
• Full Name: interleukin 33
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: NM_001164724
• Gene Symbol: IL-33
• Gene ID (NCBI): 77125
• RRID: AB_2881042
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-33 (IL-33) is a tissue-derived nuclear cytokine from the IL-1 family abundantly expressed in endothelial cells, epithelial cells and fibroblast-like cells, both during homeostasis and inflammation. IL-33 activates many immune cell types involved in type-2 immunity and allergic inflammation, including ILC2s, mast cells, Th2 cells, eosinophils, basophils, dendritic cells and alternatively activated macrophages (AAM). As a cytokine, IL-33 interacts with the receptors ST2 (also known as IL1RL1) and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. IL-33 is also effective in reversing Alzheimer-like symptoms in APP/PS1 mice, by reversing the buildup and preventing the new formation of amyloid plaques. IL-33 is synthesized as a 30-34 kD full-length form and 20 kDa form mature form.