Tested Applications
| Positive WB detected in | Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29523-1-AP targets mNeonGreen in WB, IF, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, yeast |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31617 Product name: Recombinant amphioxus lanceolatum mNeonGreen protein Source: e coli.-derived, pRSET A Tag: his Domain: 10003951 of PF01353 Sequence: Predict reactive species |
| Full Name | mNeonGreen |
| Calculated Molecular Weight | 29 kDa |
| GenBank Accession Number | PF01353 |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_3086140 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A0A1S4NYF2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
mNeonGreen is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 2013, derived from Branchiostoma lanceolatum. It is reported to be a very rapidly-maturing monomer with moderate acid sensitivity. This antibody can be used to detect the mNeonGreen tagged recombinant protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for mNeonGreen antibody 29523-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The mNeonGreen Polyclonal antibody (Cat# 29523-1-AP) has 17 citations. These appear in journals including Nature Communications, Nature Plants, PNAS, Nucleic Acids Research, Journal of Advanced Research, Cancer Cell International, mBio, Molecular Microbiology, Journal of Biochemistry, eLife, and bioRxiv. Research fields span neuroscience (synaptic plasticity), gene editing (CRISPR/base editing), RNA biology, plant development, virology, oncology (glioblastoma), microbiology (malaria, Pneumocystis), and cell biology (mitochondrial function, nuclear envelope assembly, macromolecular crowding).
| Species | Application | Title |
|---|---|---|
Nat Commun Dynamic recruitment of CaMKII into SHANK3 phase-separated condensates tunes postsynaptic density remodeling during long-term potentiation. | ||
Nat Commun Fmp30p is a mitochondrial phosphatidylinositol hydrolase that modulates CoQ biosynthesis. | ||
J Adv Res UHRF1-mediated DNA 5-mC modification drives super-enhancer redistribution and impedes osteogenesis via TGM2-regulated autophagic flux in senile osteoporosis. | ||
Nucleic Acids Res Cap-adjacent 2'-O-ribose methylation of RNA in C. elegans is required for postembryonic growth and germline development in the presence of the decapping exonuclease EOL-1. | ||
Reviews
What customers say
Users report mixed results for this mNeonGreen tag antibody. One reviewer rated it 5/5, finding it "pretty good" for immunofluorescence at 1:2000 dilution. Another gave 4/5, noting good specificity for mNeonGreen-tagged proteins in Western blot at 1:10000. However, a third reviewer rated it 3/5, observing non-specific bands in WB at 1:1000 with HEK293T lysates, though the target mNG-tagged protein was still detectable. Overall, the antibody works but may require optimization of dilution to reduce background.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jose (Verified Customer) (09-10-2026) | The antibody showed good specificity for proteins tagged with mNeongreen
|
Kai (Verified Customer) (12-23-2025) | pretty good for IF
|
Amy (Verified Customer) (03-18-2024) | Not very clean, some non-specific bands, mNG-tagged protein can be observed. HEK293T lysates 25ug.
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