Tested Applications
| Positive WB detected in | Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
29523-1-AP targets mNeonGreen in WB, IF, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31617 Product name: Recombinant amphioxus lanceolatum mNeonGreen protein Source: e coli.-derived, pRSET A Tag: his Domain: 10003951 of PF01353 Sequence: Predict reactive species |
| Full Name | mNeonGreen |
| Calculated Molecular Weight | 29 kDa |
| GenBank Accession Number | PF01353 |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_3086140 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A0A1S4NYF2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
mNeonGreen is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 2013, derived from Branchiostoma lanceolatum. It is reported to be a very rapidly-maturing monomer with moderate acid sensitivity. This antibody can be used to detect the mNeonGreen tagged recombinant protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for mNeonGreen antibody 29523-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Unlicensed origin DNA melting by MCV and SV40 polyomavirus LT proteins is independent of ATP-dependent helicase activity | ||
Elife A Tad-like apparatus is required for contact-dependent prey killing in predatory social bacteria | ||
Cancer Cell Int Regulation of microtubule nucleation in glioblastoma cells by ARF GTPase-activating proteins GIT1 and GIT2 and protein kinase C |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Amy (Verified Customer) (03-18-2024) | Not very clean, some non-specific bands, mNG-tagged protein can be observed. HEK293T lysates 25ug.
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