ChromoTek mNeonGreen Monoclonal antibody (32F6)

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins

Specificity

Spot-Tag

Applications

IF, WB

Conjugate

0.5 – 1 mg/mL (conjugates)

Publications

28

Cat no : 32f6

Synonyms

Spot tag, Spot, PDRVRAVSHWSS, Spot-Label

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Validation Data Gallery

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  SPOT tag related products

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Product Information

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins

• Applications: IF, WB
• Recommended Dilution: IF: 1:10,000; Immunoblotting: 1:1,000 to 1:20,000; Optimal working concentration is application-dependent and should be determined by testing
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 6 nM
• Specificity/Target: Spot-Tag (PDRVRAVSHWSS)
• GeneID (NCBI): 0
• Conjugate: 0.5 – 1 mg/mL (conjugates)
• Storage Buffer: Buffered aqueous solution (PBS)
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C / 40°F.Stable for 6 months. Do not freeze. Protect from light.

Publications

Application	Title
WB	PLoS Negl Trop Dis; Expanding the toolbox for Trypanosoma cruzi: A parasite line incorporating a bioluminescence-fluorescence dual reporter and streamlined CRISPR/Cas9 functionality for rapid in vivo localisation and phenotyping.; Authors - Fernanda Cristina Costa
WB	Cytoskeleton (Hoboken); Septin-associated proteins Aim44 and Nis1 traffic between the bud neck and the nucleus in the yeast Saccharomyces cerevisiae.; Authors - Adam M Perez
IHC	PLoS Genet; The Drosophila actin nucleator DAAM is essential for left-right asymmetry.; Authors - Anil Chougule
WB	AMB Express; Establishment of the monomeric yellow-green fluorescent protein mNeonGreen for life cell imaging in mycelial fungi.; Authors - Antonia Werner
WB	mBio; Z-Ring-Associated Proteins Regulate Clustering of the Replication Terminus-Binding Protein ZapT in Caulobacter crescentus.; Authors - Shogo Ozaki
IF	Cell Stem Cell; Organoid modeling of Zika and herpes simplex virus 1 infections reveals virus-specific responses leading to microcephaly.; Authors - Veronica Krenn