ChromoTek mNeonGreen Monoclonal antibody (32F6)

Mouse monoclonal antibody [32F6] to mNeonGreen fluorescent protein derived from Branchiostoma lanceolatum.

Host/IsoType

Mouse/IgG2c

Specificity

mNeonGreen

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

32F6

Publications

34

Cat no : 32f6

Synonyms

32F6, mNeonGreen, mNeonGreen tag

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Validation Data Gallery

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  Western blot analysis of cell extract from HEK293T cells transiently expressing mNeonGreen-beta-Actin (68.7 kDa).

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  Immunostaining of Drosphila embryos expressing an mNeonGreen tagged protein. Left mNeonGreen, right anti-mNeonGreen antibody 32F6. Corutesy Clara Sidor, MRC-LMB

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  Immunostaining of HeLa cells transiently expressing mNeonGreen fused to Actin Chromobody (green) with 32F6 anti-mNeonGreen antibody (red). Merge image shows overlay of green and red channels and DAPI (blus). Scale bar, 10 μm

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Product Information

Mouse monoclonal antibody [32F6] to mNeonGreen fluorescent protein derived from Branchiostoma lanceolatum.

• Applications: WB, IF, ELISA
• Recommended Dilution: Western Blot: 1:1,000; Immunofluorescence: 1:500; ELISA: 1:1,000
• Clonality: Monoclonal
• Host/IsoType: Mouse/IgG2c
• Specificity/Target: The antibody recognizes mNeonGreen at the N-terminus, C-terminus, or internal site of the fusion protein.
• Conjugate: Unconjugated
• CloneNo.: 32F6
• Storage Buffer: 0.09 % Sodium azide
• Storage Condition: Shipped at ambient temperature. Store at +4°C. Stable for one year.

Kit components

Component	Description

Publications

Application	Title
WB	PLoS Negl Trop Dis; Expanding the toolbox for Trypanosoma cruzi: A parasite line incorporating a bioluminescence-fluorescence dual reporter and streamlined CRISPR/Cas9 functionality for rapid in vivo localisation and phenotyping.; Authors - Fernanda Cristina Costa
WB	Cytoskeleton (Hoboken); Septin-associated proteins Aim44 and Nis1 traffic between the bud neck and the nucleus in the yeast Saccharomyces cerevisiae.; Authors - Adam M Perez
IHC	PLoS Genet; The Drosophila actin nucleator DAAM is essential for left-right asymmetry.; Authors - Anil Chougule
WB	AMB Express; Establishment of the monomeric yellow-green fluorescent protein mNeonGreen for life cell imaging in mycelial fungi.; Authors - Antonia Werner
WB	mBio; Z-Ring-Associated Proteins Regulate Clustering of the Replication Terminus-Binding Protein ZapT in Caulobacter crescentus.; Authors - Shogo Ozaki
IF	Cell Stem Cell; Organoid modeling of Zika and herpes simplex virus 1 infections reveals virus-specific responses leading to microcephaly.; Authors - Veronica Krenn