ChromoTek mNeonGreen Monoclonal antibody (32F6)

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins

Specificity

Spot-Tag

Applications

IF, WB

Conjugate

0.5 – 1 mg/mL (conjugates)

Cat no : 32f6

Synonyms

Spot tag, Spot, PDRVRAVSHWSS, Spot-Label



Product Information

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins

ApplicationsIF, WB
Recommended DilutionIF: 1:10,000; Immunoblotting: 1:1,000 to 1:20,000; Optimal working concentration is application-dependent and should be determined by testing
Clonality Monoclonal
HostAlpaca
AffinityDissociation constant KD of 6 nM
Specificity/TargetSpot-Tag (PDRVRAVSHWSS)
GeneID (NCBI)0
Conjugate0.5 – 1 mg/mL (conjugates)
Storage BufferBuffered aqueous solution (PBS)
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C / 40°F.Stable for 6 months. Do not freeze. Protect from light.

Publications

ApplicationTitle
WB

PLoS Negl Trop Dis

Expanding the toolbox for Trypanosoma cruzi: A parasite line incorporating a bioluminescence-fluorescence dual reporter and streamlined CRISPR/Cas9 functionality for rapid in vivo localisation and phenotyping.

Authors - Fernanda Cristina Costa
WB

Cytoskeleton (Hoboken)

Septin-associated proteins Aim44 and Nis1 traffic between the bud neck and the nucleus in the yeast Saccharomyces cerevisiae.

Authors - Adam M Perez
IHC

PLoS Genet

The Drosophila actin nucleator DAAM is essential for left-right asymmetry.

Authors - Anil Chougule
WB

AMB Express

Establishment of the monomeric yellow-green fluorescent protein mNeonGreen for life cell imaging in mycelial fungi.

Authors - Antonia Werner
WB

mBio

Z-Ring-Associated Proteins Regulate Clustering of the Replication Terminus-Binding Protein ZapT in Caulobacter crescentus.

Authors - Shogo Ozaki
IF

Cell Stem Cell

Organoid modeling of Zika and herpes simplex virus 1 infections reveals virus-specific responses leading to microcephaly.

Authors - Veronica Krenn