Validation Data Gallery
Product Information
Rabbit monoclonal antibody to mNeonGreen. Recombinant fusion of anti-mNeonGreen VHH [CTK0203] and rabbit IgG Fc domain.
| Applications | IF |
| Host/IsoType | Rabbit/IgG |
| Species Specificity | Recombinant protein from Branchiostoma lanceolatum |
| Specificity/Target | mNeonGreen fluorescent protein |
| Conjugate | Unconjugated |
| Recommended Dilution | IF/ICC: 1:200 - 1:2,000 |
| GenBank Accession Number | KC295282 |
| Purification Method | Recombinantly expressed and purified by IMAC |
| Type | Primary antibody; chimeric fusion of Nanobody (VHH) and rabbit IgG Fc domain |
| Class | Recombinant |
| Format | Recombinant monoclonal rabbit antibody |
| Immunogen | Purified recombinant mNeonGreen [Branchiostoma lanceolatum] |
| Clonality | Monoclonal |
| Clone No. | CTK0203 |
| Calculated MW | 78.1 kDa |
| Form | Buffered aqueous solution |
| RRID | AB_2889369 |
| Storage Buffer | PBS, 10% glycerol, Preservative: 0.02 % sodium azide, safety datasheet (SDS): sodium azide |
| Storage Condition | Shipped on ice. Aliquot upon receipt and store at -20°C/-4°F. Avoid freeze-thaw cycles. |
| Size | 20 µL; 100 µL |
Publications
| Application | Title |
|---|---|
Science Immotile cilia mechanically sense the direction of fluid flow for left-right determination |
![HeLa cells transiently expressing Lamin-Chromobody?-mNeonGreen were immunostained with mNeonGreen recombinant antibody, VHH-rabbit IgG Fc fusion [CTK0203] (nfrb, 1:1,000) and Nano-Secondary? alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor? 647 [CTK0101, CTK0102] (srbAF647-1, 1:1,000). Scale bar, 10 μM. HeLa cells transiently expressing Lamin-Chromobody?-mNeonGreen were immunostained with mNeonGreen recombinant antibody, VHH-rabbit IgG Fc fusion [CTK0203] (nfrb, 1:1,000) and Nano-Secondary? alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor? 647 [CTK0101, CTK0102] (srbAF647-1, 1:1,000). Scale bar, 10 μM.](https://www.ptglab.com/products/pictures/nfrb-1.jpg)