Nectin-4/PVRL4 Polyclonal antibody

Nectin-4/PVRL4 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 21903-1-AP

Synonyms

Ig superfamily receptor LNIR, LNIR, nectin 4, Nectin-4, poliovirus receptor related 4, PRR4, PVRL4

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Validation Data Gallery

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  WB analysis of MCF-7 using 21903-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21903-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 21903-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 21903-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 21903-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 21903-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 21903-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 21903-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug Nectin-4/PVRL4 antibody (21903-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, NIH/3T3 cells, A2780 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: NIH/3T3 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

21903-1-AP targets Nectin-4/PVRL4 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Nectin-4/PVRL4 fusion protein Ag14127
• Full Name: poliovirus receptor-related 4
• Calculated Molecular Weight: 510 aa, 55 kDa
• Observed Molecular Weight: 43 kDa, 66 kDa
• GenBank Accession Number: BC010423
• Gene Symbol: PVRL4
• Gene ID (NCBI): 81607
• RRID: AB_2878941
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nectin-4, or PVRL4, belongs to the family of cell adhesion molecules that regulate the formation of adherence functions in epithelial cells through the AF-6 afadin scaffold molecule. Nectin-4 is reported as a histological and serological marker for breast carcinoma. Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).

Publications

Species	Application	Title
human	WB	Mol Oncol; MiR-31 and miR-128 regulates poliovirus receptor-related 4 mediated measles virus infectivity in tumors.; Authors - Hirosha Geekiyanage
mouse	WB,IHC	Int J Urol; Chemotherapy with gemcitabine and cisplatin downregulates tumor expression level of nectin-4 in a syngeneic model of murine MBT2 urothelial cancer cell line and C3H mice; Authors - Makito Miyake
human	IHC	Oncol Lett; Upregulation of nectin-4 is associated with ITGB1 and vasculogenic mimicry and may serve as a predictor of poor prognosis in colorectal cancer.; Authors - Jinxiu Zhang
human,mouse	WB	Cell Biosci; Interferon-stimulated gene PVRL4 broadly suppresses viral entry by inhibiting viral-cellular membrane fusion; Authors - Qiaomei Cai ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Morgan (Verified Customer) (08-09-2021)

I had a lot of trouble using this PVRL4 antibody on western blots of 2 ovarian cancer cell lines; OV8 and OV90. I kept getting prominent bands at 250 kda. I tried cutting the 250 band to see if that would help, but it did not make the signal any better in the band(s) below. Any insight on this would be greatly appreciated. Thank you.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: OV8 and 0V90

Nectin-4/PVRL4 Antibody Western Blot validation (1:1000 dilution) in OV8 and 0V90 (Cat no:21903-1-AP)