Nectin-4/PVRL4 Monoclonal antibody

Nectin-4/PVRL4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D4B6

Cat no : 67721-1-Ig

Synonyms

Ig superfamily receptor LNIR, LNIR, nectin 4, Nectin-4, poliovirus receptor related 4, PRR4, PVRL4

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Validation Data Gallery

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  WB analysis of human placenta using 67721-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67721-1-Ig (Nectin-4/PVRL4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 67721-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67721-1-Ig (Nectin-4/PVRL4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human placenta tissue, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67721-1-Ig targets Nectin-4/PVRL4 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Nectin-4/PVRL4 fusion protein Ag14414
• Full Name: poliovirus receptor-related 4
• Calculated Molecular Weight: 510 aa, 55 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC010423
• Gene Symbol: Nectin-4
• Gene ID (NCBI): 81607
• RRID: AB_2882908
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion (PMID: 18703497). The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction (PMID: 11544254). It acts as a receptor for measles virus (PMID: 22048310). Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic (PMID: 35131876). Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).