SNIP/p140Cap Polyclonal antibody

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  WB analysis using 55362-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55362-1-AP (p140Cap antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55362-1-AP

  IP result of anti-SNIP/p140Cap (IP:55362-1-AP, 4ug; Detection:55362-1-AP 1:500) with mouse brain tissue lysate 4000 ug.

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  IHC staining of mouse brain using 55362-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55362-1-AP (p140Cap antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 55362-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55362-1-AP (p140Cap antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SNIP/p140Cap Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 55362-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

SNIP, SNAP25 interacting protein, SNAP 25 interacting protein, p140Cap, P140

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

55362-1-AP targets SNIP/p140Cap in WB, IHC, IP, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: SNAP25-interacting protein
• Calculated Molecular Weight: 127 kDa
• Observed Molecular Weight: 140-145 kDa
• GenBank Accession Number: NM_025248
• Gene Symbol: SNIP
• Gene ID (NCBI): 80725
• RRID: AB_2881315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9C0H9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	EBioMedicine; Genome-wide CRISPR/Cas9 screening identifies a targetable MEST-PURA interaction in cancer metastasis; Authors - Wen Wen Xu
mouse	WB	Cell Rep; The kinesin Kif21b binds myosin Va and mediates changes in actin dynamics underlying homeostatic synaptic downscaling; Authors - Kira V Gromova
mouse	WB	Mol Neurobiol; SNX17 Mediates Dendritic Spine Maturation via p140Cap; Authors - Qiuyan Cui