p14ARF/p19ARF Polyclonal antibody

p14ARF/p19ARF Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31221-1-AP

Synonyms

p14ARF, p19ARF, CDKN2A, MTS1, P16-INK4A

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Validation Data Gallery

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  WB analysis using 31221-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31221-1-AP (p14ARF/p19ARF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31221-1-AP targets p14ARF/p19ARF in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: p14ARF/p19ARF fusion protein Ag33340
• Full Name: cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
• Calculated Molecular Weight: 14kd
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: NM_058195
• Gene Symbol: CDKN2A
• Gene ID (NCBI): 1029
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDKN2A locus on chromosome 9p21 encodes two transcripts/proteins INK4A and ARF. INK4A (also referred to as p16INK4A) specially blocks the CDK4 and CDK6 cyclin-dependent kinases that control the phosphorylation status of the retinoblastoma (RB) protein (PMID: 11584300, PMID: 8259215). ARF (known as p14ARF in man and p19ARF in mouse) has been designed as a positive regulator of p53 levels because, through its complexation with Mdm2 (HDM2 in human), it prevents mdm2-mediated cytoplasmic translocation and degradation of p53 (PMID: 9153396).