p300 Recombinant monoclonal antibody

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  IP experiment of HeLa using 83078-5-RR

  IP result of anti-p300 (IP:83078-5-RR, 4ug; Detection:83078-5-RR 1:1000) with HeLa cells lysate 1050 ug.

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  IF Staining of A431 using 83078-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using p300 antibody (83078-5-RR, Clone: 230369D12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HepG2 using 83078-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p300 antibody (83078-5-RR, Clone: 230369D12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of THP-1 using 83078-5-RR

  1x10^6 THP-1 cells were intracellularly stained with 0.25 ug Anti-Human EP300 (83078-5-RR, Clone:230369D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  ChIP experiment of K-562 using 83078-5-RR

  Chromatin was prepared from K-562 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of p300 (83078-5-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

p300 Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR

Cat No. 83078-5-RR

Clone No.230369D12

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR

Synonyms

EP300, 230369D12, E1A-associated protein p300, EC:2.3.1.-, EC:2.3.1.48

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Tested Applications

• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: A431 cells, HepG2 cells
• Positive FC (Intra) detected in: THP-1 cells
• Positive ChIP-qPCR detected in: K-562 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
• CHIP-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

83078-5-RR targets p300 in WB, IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: E1A binding protein p300
• Calculated Molecular Weight: 264 kDa
• Observed Molecular Weight: 300 kDa
• GenBank Accession Number: NM_001429
• Gene Symbol: EP300
• Gene ID (NCBI): 2033
• RRID: AB_3670795
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q09472
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Death Differ; GCLC desuccinylation regulated by oxidative stress protects human cancer cells from ferroptosis; Authors - Zixiang Chen