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Various lysates were subjected to SDS PAGE followed by western blot with RMX00006 (p38 MAPK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Various lysates were subjected to SDS PAGE followed by western blot with RMX00006 (p38 MAPK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using RMX00006
IP result of anti-p38 MAPK (IP:RMX00006, 4ug; Detection:RMX00006 1:10000) with HEK-293 cells lysate 1360 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using RMX00006 (p38 MAPK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using RMX00006
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using RMX00006 (p38 MAPK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using RMX00006 (p38 MAPK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using RMX00006
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using RMX00006 (p38 MAPK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using RMX00006 (p38 MAPK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using RMX00006
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p38 MAPK antibody (RMX00006) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p38 MAPK antibody (RMX00006) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
human lung cancer tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
RMX00006 targets p38 MAPK in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.
This validation data has been generated in-house and reviewed by Proteintech scientists.
Find out more on our antibody validation policy
WB Figures
WB analysis using RMX00006
Various lysates were subjected to SDS PAGE followed by western blot with RMX00006 (p38 MAPK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse heart using RMX00006
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using RMX00006 (p38 MAPK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using RMX00006
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using RMX00006 (p38 MAPK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using RMX00006
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using RMX00006 (p38 MAPK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using RMX00006
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using RMX00006 (p38 MAPK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HEK-293 using RMX00006
IP result of anti-p38 MAPK (IP:RMX00006, 4ug; Detection:RMX00006 1:10000) with HEK-293 cells lysate 1360 ug.
IF/ICC Figures
IF Staining of HepG2 using RMX00006
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p38 MAPK antibody (RMX00006) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
p38 MAPK Multi-Recombinant antibody
Catalog Number
RMX00006
Citations
-
Dilutions
WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:200-1:800 IF/ICC : 1:500-1:2000
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
