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  • KD/KO Validated

p57Kip2 Polyclonal antibody

p57Kip2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 23317-1-AP

Synonyms

BWCR, BWS, CDKN1C, KIP2, p57, p57Kip2, WBS



Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, Jurkat cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

23317-1-AP targets p57Kip2 in WB, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p57Kip2 fusion protein Ag19849
Full Name cyclin-dependent kinase inhibitor 1C (p57, Kip2)
Calculated molecular weight 316 aa, 32 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC067842
Gene symbol CDKN1C
Gene ID (NCBI) 1028
RRIDAB_2879254
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDKN1C, also named as p57KIP2 and KIP2, belongs to the CDI family. CDKN1C is a potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. It is a negative regulator of cell proliferation. CDKN1C may play a role in maintenance of the non-proliferative state throughout life.

Protocols

Product Specific Protocols
WB protocol for p57Kip2 antibody 23317-1-APDownload protocol
IHC protocol for p57Kip2 antibody 23317-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Cancer Res

microRNA-221 Enhances MYCN via Targeting Nemo-like Kinase and Functions as an Oncogene Related to Poor Prognosis in Neuroblastoma.

Authors - Xiao-Yan He
humanWB

FASEB J

Downregulation of ALKBH5 rejuvenates aged human mesenchymal stem cells and enhances their therapeutic efficacy in myocardial infarction

Authors - Xiaoyan Gao
humanWB,IHC

Cancer Med

PIK3R3 is upregulated in liver cancer and activates Akt signaling to control cancer growth by regulation of CDKN1C and SMC1A

Authors - Weidong Lin
  • KD Validated
humanWB,CoIP,IHC

Cell Death Dis

The FOXM1/RNF26/p57 axis regulates the cell cycle to promote the aggressiveness of bladder cancer.

Authors - Lu Yi
humanWB

Oncogene

Dual network analysis of transcriptome data for discovery of new therapeutic targets in non-small cell lung cancer

Authors - Yuquan Bai