NGFR/TNFRSF16 Recombinant monoclonal antibody

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  WB analysis using 85192-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85192-1-RR (p75NTR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85192-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85192-1-RR against Rat NGFR/TNFRSF16 were performed. The affinity constant is 0.148 nM.

NGFR/TNFRSF16 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85192-1-RR

Clone No.242465H4

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor, Low affinity neurotrophin receptor p75NTR, Gp80-LNGFR, CD271

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Tested Applications

• Positive WB detected in: SW480 cells, PC-12 cells, rat uterus tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85192-1-RR targets NGFR/TNFRSF16 in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  fusion protein

• Full Name: nerve growth factor receptor (TNFR superfamily, member 16)
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 70-75 kDa
• Gene Symbol: Ngfr
• Gene ID (NCBI): 24596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P07174
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

p75NTR, also named TNFRSF16, Gp80-LNGFR, p75 ICD, and CD271, is a low-affinity receptor that can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as cell death of neural cells. The antibody is specific to NGFR.