ChromoTek pSpot-Tag-Actin vector

The plasmid pSpot-Tag®-Actin is a mammalian expression vector encoding human β-actin fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the N-terminus of β-actin. This plasmid may be used as a positive control for immunofluorescence microscopy or Western blot experiments using the Spot-Tag® system.

Vector type

Mammalian expression vector

Promoter

CMV

Reporter

Spot-Tag® N-terminal

Cat no : ev-31

Synonyms

pSpot-Tag-Actin vector, Spot vector



Product Information

The plasmid pSpot-Tag®-Actin is a mammalian expression vector encoding human β-actin fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the N-terminus of β-actin. This plasmid may be used as a positive control for immunofluorescence microscopy or Western blot experiments using the Spot-Tag® system.

DescriptionThe plasmid pSpot-Tag®-Actin is a mammalian expression vector encoding human beta-actin fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the N-terminus of β-actin. This plasmid may be used as a positive control for immunofluorescence microscopy or Western blot experiments using the Spot-Tag® system.
ApplicationsProtein expression1
Vector typeMammalian expression vector
PromoterCMV
ReplicationpMB1
ReporterSpot-Tag® (PDRVRAVSHWSS)N-terminal
Codon usageMammalian
SelectionKan/Neo

Documentation

SDS
?SDS.pdf
Datasheet
ev-31.pdf