ChromoTek pSpot3 vector

The plasmid pSpot3 is an expression vector for Spot-Tag® fusion proteins in E. coli. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot3, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the C-terminus of the POI.

Vector type

bacterial expression vector

Promoter

T7

Reporter

Spot-Tag® C-terminal

Cat no : ev-3

Synonyms

pSpot3 vector, Spot vector

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Validation Data Gallery

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  T7 promoter: 1-19 Lac operon: 19-46 RBS: 64-80 MCS: 86-140 Spot-Tag®: 141-176 Stop codon (TAA): 177-179 rrnB terminator: 361-507 Ampicillin resistance gene: 955-1815 pRB322 replication origin: 1970-2589 Lac operon: 2895-2922

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  Multiple cloning site (MCS)

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Product Information

The plasmid pSpot3 is an expression vector for Spot-Tag® fusion proteins in E. coli. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot3, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the C-terminus of the POI.

• Applications: Protein expression
• Vector type: bacterial expression vector
• Promoter: T7
• Replication: pBR322
• Reporter: Spot-Tag® (PDRVRAVSHWSS) C-terminal
• Selection: ampicillin