ChromoTek pSpot4 vector

Validation Data Gallery

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  T7 promoter: 1-19 Lac operon: 18-46 RBS: 64-80 Start codon (ATG): 88-90 Spot-Tag®: 91-126 MCS: 127-172 rrnB terminator: 364-510 Ampicillin resistance gene: 958-1818 pRB322 replication origin: 1973-2592 Lac operon: 2898-2925

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  Multiple cloning site (MCS)

The plasmid pSpot4 is an expression vector for Spot-Tag® fusion proteins in E. coli. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot4, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.

Cat No. ev-4

Vector type

bacterial expression vector

Promoter

T7

Reporter

Spot-Tag® N-terminal

Synonyms

pSpot4 vector, Spot vector

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Product Information

The plasmid pSpot4 is an expression vector for Spot-Tag® fusion proteins in E. coli. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot4, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.

• Applications: Protein expression
• Vector type: bacterial expression vector
• Promoter: T7
• Replication: pBR322
• Reporter: Spot-Tag® (PDRVRAVSHWSS) N-terminal
• Selection: ampicillin

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