ChromoTek pSpot8 GFP-Spot-Tag

Validation Data Gallery

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  TPI1 promoter: 9576-9 GFPuv-Spot-Tag®: 10-780 Spot-Tag®: 742-777 T7 primer site: 892-910 Leucin marker (LEU2): 2226-3332 2µ replication origin: 4058-5527 Ampicillin resistance gene: 6385-7245 pMB1 replication origin: 7400-8019

The plasmid pSpot8_GFP-Spot-Tag is a S. cerevisiae expression vector encoding the Aequorea victoria Green fluorescent protein (GFP) variant GFPuv fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the C-terminus of GFPuv. This plasmid may be used as a positive control for immunoprecipitation or Western blot experiments using the Spot-Tag® system.

Cat No. ev-33

Vector type

Yeast expression vector

Promoter

TPI1

Reporter

Spot-Tag® C-termina

Synonyms

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Product Information

The plasmid pSpot8_GFP-Spot-Tag is a S. cerevisiae expression vector encoding the Aequorea victoria Green fluorescent protein (GFP) variant GFPuv fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the C-terminus of GFPuv. This plasmid may be used as a positive control for immunoprecipitation or Western blot experiments using the Spot-Tag® system.

• Description: The plasmid pSpot8_GFP-Spot-Tag is a S. cerevisiae expression vector encoding the Aequorea victoria Green fluorescent protein (GFP) variant GFPuv fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the C-terminus of GFPuv. This plasmid may be used as a positive control for immunoprecipitation or Western blot experiments using the Spot-Tag® system.
• Applications: Protein expression
• Vector type: Yeast expression vector
• Promoter: TPI1
• Replication: 2µ (S. cerevisiae) pBR322 (E. coli)
• Reporter: Spot-Tag® (PDRVRAVSHWSS) C-termina
• Selection: Leucin (S. cerevisiae) Ampicillin (E. coli)

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