ChromoTek pSpot8 vector

The plasmid pSpot8 is an expression vector for Spot-Tag® fusion proteins in S. cerevisiae. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot8, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the C-terminus of the POI.

Vector type

yeast expression vector

Promoter

TPI1

Reporter

Spot-Tag® C-terminal

Cat no : ev-8

Synonyms

pSpot8 vector, Spot vector



Product Information

The plasmid pSpot8 is an expression vector for Spot-Tag® fusion proteins in S. cerevisiae. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot8, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the C-terminus of the POI.

ApplicationsProtein expression
Vector typeyeast expression vector
PromoterTPI1
Replication2µ (S. cerevisiae) pBR322 (E. coli)
ReporterSpot-Tag® (PDRVRAVSHWSS) C-terminal
Selectionleucin (S. cerevisiae) ampicillin (E. coli)

Documentation

SDS
SDS.pdf
Datasheet
ev-8.pdf