|Positive WB detected in||SKOV-3 cells, A2780 cells|
|Positive IP detected in||SKOV-3 cells|
|Positive IHC detected in||human ovary tumor tissue, human renal cell carcinoma tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SKOV-3 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21383-1-AP is Pan-PAX Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Pan-PAX fusion protein Ag16018|
|Full Name||paired box 8|
|Calculated molecular weight||450 aa, 48 kDa|
|Observed molecular weight||36-48 kDa|
|GenBank accession number||BC001060|
|Gene ID (NCBI)||7849|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PAX genes encode a family of developmentally regulated transcription factors that have been implicated in a number of human and murine congenital disorders, as well as in tumorigenesis. These genes are defined by the presence of an evolutionarily conserved DNA binding domain, termed the paired domain. Many paired domain-containing proteins recognize a common sequence motif. The immunogen of this atnibody is the Paired box domain of PAX family. This antibody is a Pan-PAX antibody.
|Product Specific Protocols|
|WB protocol for Pan-PAX antibody 21383-1-AP||Download protocol|
|IHC protocol for Pan-PAX antibody 21383-1-AP||Download protocol|
|IF protocol for Pan-PAX antibody 21383-1-AP||Download protocol|
|IP protocol for Pan-PAX antibody 21383-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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PAX8 and PAX2 immunostaining facilitates the diagnosis of primary epithelial neoplasms of the male genital tract.
Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn.