Validation Data Gallery
|Positive WB detected in
|SKOV-3 cells, A2780 cells
|Positive IP detected in
|Positive IHC detected in
|human ovary tumor tissue, human renal cell carcinoma tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
The immunogen of 21383-1-AP is Pan-PAX Fusion Protein expressed in E. coli.
|human, mouse, rat
|human, mouse, rat, pig
|Host / Isotype
|Rabbit / IgG
|Pan-PAX fusion protein Ag16018
|paired box 8
|Calculated molecular weight
|450 aa, 48 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
PAX genes encode a family of developmentally regulated transcription factors that have been implicated in a number of human and murine congenital disorders, as well as in tumorigenesis. These genes are defined by the presence of an evolutionarily conserved DNA binding domain, termed the paired domain. Many paired domain-containing proteins recognize a common sequence motif. The immunogen of this atnibody is the Paired box domain of PAX family. This antibody is a Pan-PAX antibody.
|Product Specific Protocols
|WB protocol for Pan-PAX antibody 21383-1-AP
|IHC protocol for Pan-PAX antibody 21383-1-AP
|IF protocol for Pan-PAX antibody 21383-1-AP
|IP protocol for Pan-PAX antibody 21383-1-AP
|Click here to view our Standard Protocols
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PAX8 and PAX2 immunostaining facilitates the diagnosis of primary epithelial neoplasms of the male genital tract.