Phospho-Marcks Antibody

Phospho-Marcks Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 10018-3-AP

Synonyms

Macs, Marcks, phospho-Marcks, PKCSL



Tested Applications

Positive WB detected inMouse J774 Macrophage cellss-treated with PMA, HeLa cells, Mouse J774 Macrophage cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

10018-3-AP targets Phospho-Marcks in WB, ELISA applications and shows reactivity with human, mouse samples.

Reactivity human, mouse
Cited Species bovine, human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myristoylated alanine rich protein kinase C substrate
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession number BC046601
Gene symbol Marcks
Gene ID (NCBI) 17118
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. This antibody is raised against phospho-Marcks with S152/S163 phosphorylated and phospho-Marcksl1 (geneID:17357) with S93/S104 phosphorylated.

Protocols

Product Specific Protocols
WB protocol for Phospho-Marcks antibody 10018-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Open Virol J

HIV-1 Antigens in Neurons of Cocaine-Abusing Patients.

Authors - Fiala Milan M
ratWB

Am J Physiol Cell Physiol

Involvement of PKCdelta and PKD in pulmonary microvascular endothelial cell hyperpermeability.

Authors - Tinsley John H JH
ratWB

J Physiol

5-HT evokes sensory long-term facilitation of rodent carotid body via activation of NADPH oxidase.

Authors - Peng Ying-Jie YJ
ratWB

J Neurosci

NADPH oxidase is required for the sensory plasticity of the carotid body by chronic intermittent hypoxia.

Authors - Peng Y-J YJ
bovineWB

Proc Natl Acad Sci U S A

MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability.

Authors - Jin Benjamin Y BY