|Positive WB detected in||Mouse J774 Macrophage cellss-treated with PMA, HeLa cells, Mouse J774 Macrophage cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Sample-dependent, check data in validation data gallery|
10018-3-AP targets Phospho-Marcks in WB,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||bovine, human, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||myristoylated alanine rich protein kinase C substrate|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||32 kDa|
|GenBank accession number||BC046601|
|Gene ID (NCBI)||17118|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. This antibody is raised against phospho-Marcks with S152/S163 phosphorylated and phospho-Marcksl1 (geneID:17357) with S93/S104 phosphorylated.
Open Virol J
HIV-1 Antigens in Neurons of Cocaine-Abusing Patients.
Am J Physiol Cell Physiol
Involvement of PKCdelta and PKD in pulmonary microvascular endothelial cell hyperpermeability.
5-HT evokes sensory long-term facilitation of rodent carotid body via activation of NADPH oxidase.
NADPH oxidase is required for the sensory plasticity of the carotid body by chronic intermittent hypoxia.
Proc Natl Acad Sci U S A
MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability.