HumanKine® recombinant human FGF-1 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.5-2.5 ng/mL EC50

Species Reactivity

human

Purity

> 95%

Cat no : HZ-1327

Synonyms

Acidic Fibroblast Growth Factor 1, Heparin-Binding growth Factor 1, FGF-acidic, ECGF-beta, HBGF-1, FGF-1, Fibroblast Growth Factor Acidic

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Validation Data Gallery

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  Purity of recombinant human FGF-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human FGF-1 (HZ-1327) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGF-1 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.5-2.5 ng/mL.

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  Proteintech FGF-1 demonstrates greater induction of proliferation and equivalent EC50 to leading competitors. Recombinant human FGF-1 (HZ-1327) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGFbasic-TS from either a leading competitor or Proteintech for 48 hours.

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Technical Specifications

• GeneID: 2246
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.5-2.5 ng/mL EC50
• Purity: > 95%
• Endotoxin: <1 EU/µg
• Accession Number: C9JDC5
• Molecular Mass: 18 kDa reduced, 16 kDa non-reduced, monomer, non-glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

FGF-1 is a non-glycosylated and single polypeptide protein that is a well-classified member of the fibroblast growth factor (FGF) superfamily. It stimulates DNA synthesis and proliferation in a wide range of cells and is also involved in promoting angiogenesis and wound healing processes. It is capable of binding with all four FGFRs and exerts its intracellular effects through downstream PLCγ and MAPK pathways. Dysregulated FGF-1 signaling has been associated with tumor invasion and metastasis. It is also capable of inducing apoptosis in response to vascular injury and may have use as a potential therapeutic for cardiovascular diseases (PMID: 15469994, 24829797).

Synonyms

Acidic Fibroblast Growth Factor 1, Heparin-Binding growth Factor 1, FGF-acidic, ECGF-beta, HBGF-1, FGF-1, Fibroblast Growth Factor Acidic