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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human VEGF121 protein- GMP grade

EC50

1.5-9 ng/mL EC50

Species Reactivity

human,pig

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1204-GMP

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGF121, VEGFA, VPF

Validation Data Gallery

  • Purity of GMP recombinant human VEGF121 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of GMP recombinant human VEGF121 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP Recombinant human GMP VEGF121 (HZ-1204-GMP) induces dose-dependent luciferase production in a HEK293T VEGF luciferase reporter cell line. Luciferase production was assessed by the One-Step™ luciferase assay Kit. HEK293T VEGF luciferase reporter cells were treated with increasing concentrations of GMP recombinant VEGF121 for 18 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 1.5-9 ng/mL

    GMP Recombinant human GMP VEGF121 (HZ-1204-GMP) induces dose-dependent luciferase production in a HEK293T VEGF luciferase reporter cell line. Luciferase production was assessed by the One-Step™ luciferase assay Kit. HEK293T VEGF luciferase reporter cells were treated with increasing concentrations of GMP recombinant VEGF121 for 18 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 1.5-9 ng/mL

Technical Specifications

GeneID 7422
Species Human
Expression HEK293
EC50 1.5-9 ng/mL EC50
Purity >95%
Endotoxin<0.1 EU/μg
Accession NumberP15692
Molecular Mass 17 and 21 kDa reduced, 28 to 40 kDa non-reduced, homodimer and homotrimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,pig

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulphide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells and is essential for physiological and pathological angiogenesis. Misregulation of this gene in mice results in abnormal embryonic blood vessel formation (PMID:10838264; 11329058).

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGF121, VEGFA, VPF