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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human Activin A protein

Activity

0.5-3.5 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1138

Synonyms

Activin A, Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, inhibin, beta A

Validation Data Gallery

  • The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

    The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

  • Recombinant human Activin A (HZ-1138) causes dose-dependent inhibition of proliferation in the Mouse plasmacytoma (MPC-11) cell line. Proliferation of the MPC-11 cell line was assessed using Promega CellTiter 96®. MPC-11 cells were treated with increasing concentrations of recombinant human Activin A for 72 hrs hours before addition of CellTiter96® reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.5-3.5 ng/ml.

    Recombinant human Activin A (HZ-1138) causes dose-dependent inhibition of proliferation in the Mouse plasmacytoma (MPC-11) cell line. Proliferation of the MPC-11 cell line was assessed using Promega CellTiter 96®. MPC-11 cells were treated with increasing concentrations of recombinant human Activin A for 72 hrs hours before addition of CellTiter96® reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.5-3.5 ng/ml.

Technical Specifications

GeneID 3624
Species human
Expression HEK293
Activity 0.5-3.5 ng/mL EC50
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP08476
Molecular Mass 25 kDa, homodimer, non-glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Activin A, a member of the TGF-β superfamily, was named for its ability to activate FSH secretion from the anterior pituitary gland (PMID 16885530). Three activins have been identified in human tissues: activin A, activin B, and activinAB, of which activin A is the most studied. Activin A has various functions in regulating cell differentiation, proliferation, apoptosis and carcinogenesis (PMID 8200957). Xeno-free Recombinant Human Activin A is expressed from human 293 cells as disulfide linked homodimers with molecular mass of 25 kDa.

Synonyms

Activin A, Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, inhibin, beta A

Publications

SpeciesTitle
human

Protein Cell

Modeling CADASIL vascular pathologies with patient-derived induced pluripotent stem cells.

Authors - Chen Ling
View Article
human

Tissue Eng Part C Methods

Essential components for ex vivo proliferation of mesenchymal stromal cells.

Authors - NatalieFekete
View Article
human

Cell Rep

Genome-wide R-loop Landscapes during Cell Differentiation and Reprogramming.

Authors - Pengze Yan
View Article
human

STAR Protoc

Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells.

Authors - Pooja Hor
View Article
mouse

Birth Defects Res B Dev Reprod Toxicol

Lost in translation: The search for an in vitro screen for spermatogenic toxicity.

Authors - Robert EChapin
View Article
human

Eur J Obstet Gynecol Reprod Biol

Effects of gremlin-2 on the transition of primordial follicles during early folliculogenesis in the human ovary.

Authors - YukiIkeda
View Article