HumanKine® recombinant human Activin A protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity

Typically ≤ 5 ng/mL EC50

Species Reactivity

human

Purity

>95%

Publications

22

Cat no : HZ-1138

Synonyms

Activin A, Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, inhibin, beta A

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Product data gallery View All

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  The activity was determined by the dose-dependent inhibition of proliferation of the MPC-11 cell line (mouse plasmacytoma cell line) using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

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  The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

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Technical Specifications

• GeneID: 3624
• Species: human
• Expression: HEK293
• Activity: Typically ≤ 5 ng/mL EC50
• Purity: >95%
• Molecular Mass: 25 kDa, homodimer, non-glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human, mouse

Stability and Reconstitution

Stability and Storage	Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20^oC) and (-80^oC). Upon reconstitution we recommend that the solution can be stored at (4^oC) for short term or at (-20^oC) to (-80^oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution	Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Whitepapers

Humankine Activin A has authentic protein maturation and high activity

Background

Activin A, a member of the TGF-β superfamily, was named for its ability to activate FSH secretion from the anterior pituitary gland (PMID 16885530). Three activins have been identified in human tissues: activin A, activin B, and activinAB, of which activin A is the most studied. Activin A has various functions in regulating cell differentiation, proliferation, apoptosis and carcinogenesis (PMID 8200957). Xeno-free Recombinant Human Activin A is expressed from human 293 cells as disulfide linked homodimers with molecular mass of 25 kDa.

Synonyms

Activin A, Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, inhibin, beta A

Publications

Species	Title
human	Protein Cell; Modeling CADASIL vascular pathologies with patient-derived induced pluripotent stem cells.; Authors - Chen Ling
human	Tissue Eng Part C Methods; Essential components for ex vivo proliferation of mesenchymal stromal cells.; Authors - NatalieFekete
human	Cell Rep; Genome-wide R-loop Landscapes during Cell Differentiation and Reprogramming.; Authors - Pengze Yan
human	STAR Protoc; Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells.; Authors - Pooja Hor
mouse	Birth Defects Res B Dev Reprod Toxicol; Lost in translation: The search for an in vitro screen for spermatogenic toxicity.; Authors - Robert EChapin
human	Eur J Obstet Gynecol Reprod Biol; Effects of gremlin-2 on the transition of primordial follicles during early folliculogenesis in the human ovary.; Authors - YukiIkeda

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