HumanKine® recombinant human FGFbasic-TS protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.4-2.5 ng/mL EC50 in NIH3T3 cells in defined Media.

Specific Activity

>1.00 x 10⁷ IU/mg

Species Reactivity

human,mouse,rat

Purity

>95%

Publications

47

Reviews (3)

Cat no : HZ-1285

Synonyms

Basic fibroblast growth factor, BFGF, FGF basic, FGF basic-TS, FGF2, FGFB, FGFbasic, FGFbasic-TS, HBGF 2

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Validation Data Gallery

• 

  Purity of recombinant human FGFbasic-TS was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

• 

  Recombinant human FGFbasic-TS (HZ-1285) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGFbasic-TS for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.05-0.4 ng/mL.

• 

  Recombinant human FGFbasic-TS (HZ-1285) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.02% FBS during treatment with increasing concentrations of recombinant human FGF basic-TS for 72hrs in defined medium. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.4-2.5 ng/mL.

• 

  Proteintech FGF basic-TS (HZ-1285) demonstrates greater induction of proliferation and 3-fold lower EC50 compared to leading competitors. Recombinant human FGFbasic-TS stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGFbasic-TS for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.05-0.4 ng/mL.

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Technical Specifications

• GeneID: 2247
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.4-2.5 ng/mL EC50 in NIH3T3 cells in defined Media.
• Activity(Secondary): 0.05-0.4 ng/mL EC50 in HDFa cells
• Specific Activity: >1.00 x 10^7 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P09038
• Molecular Mass: 17 kDa, monomer, non-glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse,rat

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Whitepapers

Thermostable FGF Basic Supports a 2-Day Feeding Schedule As Shown by Biochemical and Cell Culture Analysis

Background

Basic Fibroblast growth factor (FGFbasic), also known as bFGF, FGF2, FGF-β or HBGF-2, belongs to the FGF family. FGFbasic is involved in a number of biological processes including embryonic development and differentiation, neuron differentiation, survival, and regeneration, and the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin (PMID 3272178, 3316527). FGFbasic is critical for human embryonic stem cells to remain in an undifferentiated state during cell culture (PMID 15782187).

Synonyms

Basic fibroblast growth factor, BFGF, FGF basic, FGF basic-TS, FGF2, FGFB, FGFbasic, FGFbasic-TS, HBGF 2

Publications

Species	Title
human	Nat Biotechnol; An in vivo model of functional and vascularized human brain organoids.; Authors - Abed AlFatah Mansour
human	Cell Stem Cell; A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells.; Authors - Haibin Xi
human	Cell Res; Visualization of aging-associated chromatin alterations with an engineered TALE system.; Authors - Ruotong Ren
human	Mol Psychiatry; Sensing serotonin secreted from human serotonergic neurons using aptamer-modified nanopipettes.; Authors - Nako Nakatsuka
mouse	J Clin Invest; Mature myelin maintenance requires Qki to coactivate PPARβ-RXRα-mediated lipid metabolism.; Authors - Xin Zhou
mouse	Nat Commun; Qki activates Srebp2-mediated cholesterol biosynthesis for maintenance of eye lens transparency.; Authors - Seula Shin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ryszard (Verified Customer) (08-01-2022)

I tried Your HumanKine FGFbasic-TS in my culture and my cells are very happy. It's an excellent alternative to other FGFs from other companies that have trouble delivering on time their orders.

• Applications: Cell culture
• Cell Tissue Type: Neural Stem Cell Cultures

FH

Alessandro (Verified Customer) (07-27-2022)

Reliable supplement for dopaminergic neurons maturation

• Applications: Cell culture
• Cell Tissue Type: Dopaminergic Neurons

FH

Ana (Verified Customer) (03-16-2022)

Works well

• Applications: Cell culture