HumanKine® recombinant human FGFbasic-TS protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity

0.05-0.4 ng/mL EC50

Species Reactivity

Human

Purity

>95%

Publications

28

Cat no : HZ-1285

Synonyms

Basic fibroblast growth factor, BFGF, FGF basic, FGF basic-TS, FGF2, FGFB, FGFbasic, FGFbasic-TS, HBGF 2

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Validation Data Gallery

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  The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

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  Recombinant human FGFbasic-TS (HZ-1285) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGFbasic-TS for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.05-0.4 ng/mL.

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Technical Specifications

• GeneID: 2247
• Species: Human
• Expression: HEK293
• Activity: 0.05-0.4 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P09038
• Molecular Mass: 17 kDa, monomer, non-glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution	Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Whitepapers

Thermostable FGF Basic Supports a 2-Day Feeding Schedule As Shown by Biochemical and Cell Culture Analysis

Background

Basic Fibroblast growth factor (FGFbasic), also known as bFGF, FGF2, FGF-β or HBGF-2, belongs to the FGF family. FGF-basic is involved in a number of biological processes including embryonic development and differentiation, neuron differentiation, survival, and regeneration, and the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin (PMID 3272178, 3316527). FGF-basic is critical for human embryonic stem cells to remain in an undifferentiated state during cell culture (PMID 15782187).

Synonyms

Basic fibroblast growth factor, BFGF, FGF basic, FGF basic-TS, FGF2, FGFB, FGFbasic, FGFbasic-TS, HBGF 2

Publications

Species	Title
human	PLoS Biol; Up-regulation of FOXD1 by YAP alleviates senescence and osteoarthritis.; Authors - Lina Fu
human	Protein Cell; Telomere-dependent and telomere-independent roles of RAP1 in regulating human stem cell homeostasis.; Authors - Xing Zhang
human	Cell Stem Cell; FOXO3-Engineered Human ESC-Derived Vascular Cells Promote Vascular Protection and Regeneration.; Authors - Pengze Yan
human	Int J Mol Sci; β3-Adrenoreceptor Blockade Reduces Hypoxic Myeloid Leukemic Cells Survival and Chemoresistance.; Authors - Maura Calvani
human	Oxid Med Cell Longev; Autophagy Contributes to the Maintenance of Genomic Integrity by Reducing Oxidative Stress.; Authors - Wenqing Bu
	STAR Protoc; Generation of PAX7 Reporter Cells to Investigate Skeletal Myogenesis from Human Pluripotent Stem Cells.; Authors - Haibin Xi