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Animal-free Recombinant Human FGFBasic-TS3 Publications

Catalog number: HZ-1285

HEK293 expressed Endotoxin-free Animal-component free

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Format: Lyophilized

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Technical Specifications

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FGFBasic-TS activity assay

The activity was determined by the dose- dependent stimulation of the proliferation of the Balb/c 3T3 cell line using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay

FGFBasic-TS purity

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue. R represents reducing conditions and NR represents non-reducing conditions.

GeneID:
2247
Species:
Human
Expression:
HEK293
Activity:
Typically ≤0.5 ng/mL EC50
Purity:
>95%
Molecular Mass:
17 kDa, monomer, non-glycosylated
Formulation
1x PBS, See Certificate of Analysis for details
Species Reactivity:
human,  mouse

Stability and Reconstitution

Stability and Storage:
Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.


Reconstitution:
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Product Description

Product Description:

Recombinant human FGFbasic-TS™ is expressed in human 293 cells. FGFbasic is a heparin-binding growth factor and critical for human embryonic stem cells to remain in an undifferentiated state during cell culture. FGFbasic-TS™ is a protein engineered FGFbasic to enchance its thermo- stability without modification of biological function. FGFbasic-TS™ consists of 154 amino acids as a 17 kDa, non-glycosylated monomer.



Background:

Basic Fibroblast growth factor (FGFbasic), also known as bFGF, FGF2, FGF-β or HBGF-2, belongs to the FGF family. FGF-basic is involved in a number of biological processes including embryonic development and differentiation, neuron differentiation, survival, and regeneration, and the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin (PMID 3272178, 3316527). FGF-basic is critical for human embryonic stem cells to remain in an undifferentiated state during cell culture (PMID 15782187).



Synonyms:
Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin FGFbasicTS, FGFbasic TS, FGF2, FGF 2, HBGF2, HBGF, FGFbasic-TS



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