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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FGFbasic-TS protein

Activity

0.05-0.4 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1285

Synonyms

Basic fibroblast growth factor, BFGF, FGF basic, FGF basic-TS, FGF2, FGFB, FGFbasic, FGFbasic-TS, HBGF 2

Validation Data Gallery

  • The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

    The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

  • Recombinant human FGFbasic-TS (HZ-1285) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGFbasic-TS for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.05-0.4 ng/mL.

    Recombinant human FGFbasic-TS (HZ-1285) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGFbasic-TS for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.05-0.4 ng/mL.

Technical Specifications

GeneID 2247
Species Human
Expression HEK293
Activity 0.05-0.4 ng/mL EC50
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP09038
Molecular Mass 17 kDa, monomer, non-glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Basic Fibroblast growth factor (FGFbasic), also known as bFGF, FGF2, FGF-β or HBGF-2, belongs to the FGF family. FGF-basic is involved in a number of biological processes including embryonic development and differentiation, neuron differentiation, survival, and regeneration, and the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin (PMID 3272178, 3316527). FGF-basic is critical for human embryonic stem cells to remain in an undifferentiated state during cell culture (PMID 15782187).

Synonyms

Basic fibroblast growth factor, BFGF, FGF basic, FGF basic-TS, FGF2, FGFB, FGFbasic, FGFbasic-TS, HBGF 2

Publications

SpeciesTitle
human

PLoS Biol

Up-regulation of FOXD1 by YAP alleviates senescence and osteoarthritis.

Authors - Lina Fu
View Article
human

Protein Cell

Telomere-dependent and telomere-independent roles of RAP1 in regulating human stem cell homeostasis.

Authors - Xing Zhang
View Article
human

Cell Stem Cell

FOXO3-Engineered Human ESC-Derived Vascular Cells Promote Vascular Protection and Regeneration.

Authors - Pengze Yan
View Article
human

Int J Mol Sci

β3-Adrenoreceptor Blockade Reduces Hypoxic Myeloid Leukemic Cells Survival and Chemoresistance.

Authors - Maura Calvani
View Article
human

Oxid Med Cell Longev

Autophagy Contributes to the Maintenance of Genomic Integrity by Reducing Oxidative Stress.

Authors - Wenqing Bu
View Article

STAR Protoc

Generation of PAX7 Reporter Cells to Investigate Skeletal Myogenesis from Human Pluripotent Stem Cells.

Authors - Haibin Xi
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Ryszard (Verified Customer) (08-01-2022)

I tried Your HumanKine FGFbasic-TS in my culture and my cells are very happy. It's an excellent alternative to other FGFs from other companies that have trouble delivering on time their orders.

  • Applications: Cell culture
  • Cell Tissue Type: Neural Stem Cell Cultures
FH

Alessandro (Verified Customer) (07-27-2022)

Reliable supplement for dopaminergic neurons maturation

  • Applications: Cell culture
  • Cell Tissue Type: Dopaminergic Neurons
FH

Ana (Verified Customer) (03-16-2022)

Works well

  • Applications: Cell culture