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Animal-free Recombinant Human FLT3 Ligand10 Publications

Catalog number: HZ-1151

HEK293 expressed Endotoxin-free Animal-component free

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Format: Lyophilized

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Technical Specifications

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FLT3 Ligand activity assay

The activity was determined by the dose- dependent stimulation of the proliferation of the human acute myeloid leukemia cell line OCI-AML5 using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

FLT3 Ligand purity

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue. R represents reducing conditions and NR represents non-reducing conditions.

GeneID:
2323
Species:
Human
Expression:
HEK293
Activity:
Typically ≤0.8 ng/mL EC50
Purity:
>95%
Molecular Mass:
24 to 30 kDa, monomer, glycosylated
Formulation
1x PBS, See Certificate of Analysis for details
Species Reactivity:
human, mouse

Stability and Reconstitution

Stability and Storage:
Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.


Reconstitution:
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA)

Product Description

Product Description:

Animal-free Recombinant Human FLT3 Ligand is expressed from human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 24 to 30 kDa. This cytokine is produced in a serum-free, chemically defined media. The molecular mass is a narrower range than reported when this cytokine is expressed in insect Sf21 cells (17 to 30 kDa), emphasizing the difference in glycosylation in different expression systems. FLT3 Ligand is a growth factor that regulates proliferation of early hematopoietic cells. FLT3 Ligand binds to cells expressing the tyrosine kinase receptor FLT3. FLT3 Ligand by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation.



Background:

FMS-related tyrosine kinase 3 ligand (FLT3 Ligand) is a growth factor that regulates proliferation of early hematopoietic cells. FLT3 Ligand binds to cells expressing the tyrosine kinase receptor FLT3. FLT3 Ligand by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation (PMID PMID: 8590775; 7544638; 12176867).



Synonyms:
Fms-related tyrosine kinase 3 ligand Fms-related tyrosine kinase3 ligand, Fms-related tyrosine kinase-3 ligand



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