HumanKine® recombinant human FLT3 Ligand protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity

0.4-3.0 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

Publications

14

Cat no : HZ-1151

Synonyms

FL, Flt3 ligand, Flt3L, FLT3LG, SL cytokine

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Validation Data Gallery

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  Purity of recombinant human FLT3 Ligand was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human FLT3 (HZ-1151) Ligand stimulates dose-dependent proliferation of the human acute myeloid leukemia cell line (OCI-AML5). Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. OCI-AML5 cells were treated with increasing concentrations of recombinant human FLT3 Ligand for 72 hours. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range is 0.4-3 ng/mL.

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Technical Specifications

• GeneID: 2323
• Species: Human
• Expression: HEK293
• Activity: 0.4-3.0 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P49771
• Molecular Mass: 24 to 30 kDa, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA)

Background

FMS-related tyrosine kinase 3 ligand (FLT3 Ligand) is a growth factor that regulates proliferation of early hematopoietic cells. FLT3 Ligand binds to cells expressing the tyrosine kinase receptor FLT3. FLT3 Ligand by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation (PMID: 8590775; 7544638; 12176867).

Synonyms

FL, Flt3 ligand, Flt3L, FLT3LG, SL cytokine

Publications

Species	Title
human	Haematologica; CD123 expression patterns and selective targeting with a CD123-specific antibody-drug conjugate (IMGN632) in acute lymphoblastic leukemia.; Authors - Evgeniya Angelova
human	Blood Adv; A CD123-targeting antibody-drug conjugate, IMGN632, designed to eradicate AML while sparing normal bone marrow cells.; Authors - Yelena Kovtun
human	ACS Biomater Sci Eng; Bioprinting of Human Cord Blood-Derived CD34+ Cells and Exploration of the Multilineage Differentiation Ability in Vitro.; Authors - Lidan Chen
human	Blood Res; Generation of hematopoietic stem cells from human embryonic stem cells using a defined, stepwise, serum-free, and serum replacement-free monolayer culture method.; Authors - So-JungKim
human	PLoS One; A synthetic model of human beta-thalassemia erythropoiesis using CD34+ cells from healthy adult donors.; Authors - Y TerryLee
human	Sci Rep; A 3D engineered scaffold for hematopoietic progenitor/stem cell co-culture in vitro.; Authors - Dezhi Zhou