- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human FLT3 Ligand protein
Activity
0.4-3.0 ng/mL EC50
Species Reactivity
human,mouse
Purity
>95%
Cat no : HZ-1151
Synonyms
FL, Flt3 ligand, Flt3L, FLT3LG, SL cytokine
Validation Data Gallery
Technical Specifications
GeneID | 2323 |
Species | Human |
Expression | HEK293 |
Activity | 0.4-3.0 ng/mL EC50 |
Purity | >95% |
Endotoxin | <1 EU/µg |
Accession Number | P49771 |
Molecular Mass | 24 to 30 kDa, monomer, glycosylated |
Formulation | 1x PBS, See Certificate of Analysis for details |
Species Reactivity | human,mouse |
Stability and Reconstitution
Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
---|---|---|---|
Lyophilized | -20°C to -80°C | Until Expiry Date | |
Lyophilized | Room Temperature | 2 weeks | |
Reconstituted as per CofA | -20°C to -80°C | 6 months | |
Reconstituted as per CofA | 4°C | 1 week | |
Avoid repeated freeze-thaw cycles. |
Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA) |
Background
FMS-related tyrosine kinase 3 ligand (FLT3 Ligand) is a growth factor that regulates proliferation of early hematopoietic cells. FLT3 Ligand binds to cells expressing the tyrosine kinase receptor FLT3. FLT3 Ligand by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation (PMID: 8590775; 7544638; 12176867).
Synonyms
FL, Flt3 ligand, Flt3L, FLT3LG, SL cytokine
Publications
Species | Title |
---|---|
Haematologica CD123 expression patterns and selective targeting with a CD123-specific antibody-drug conjugate (IMGN632) in acute lymphoblastic leukemia. | |
Blood Adv A CD123-targeting antibody-drug conjugate, IMGN632, designed to eradicate AML while sparing normal bone marrow cells. | |
ACS Biomater Sci Eng Bioprinting of Human Cord Blood-Derived CD34+ Cells and Exploration of the Multilineage Differentiation Ability in Vitro. | |
Blood Res Generation of hematopoietic stem cells from human embryonic stem cells using a defined, stepwise, serum-free, and serum replacement-free monolayer culture method. | |
PLoS One A synthetic model of human beta-thalassemia erythropoiesis using CD34+ cells from healthy adult donors. | |
Sci Rep A 3D engineered scaffold for hematopoietic progenitor/stem cell co-culture in vitro. |