• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human GM-CSF protein

Activity

0.08-0.8 ng/mL EC50

Specific Activity

6.64x10^6 IU/mg calibrated against GM-CSF reference standard (NIBSC cat no: 88/646). Reference use only. Specific activity is not routinely tested

Species Reactivity

human,mouse,pig

Purity

>95%

Cat no : HZ-1002

Synonyms

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim



Technical Specifications

GeneID 1437
Species Human
Expression HEK293
Activity 0.08-0.8 ng/mL EC50
Specific Activity6.64x10^6 IU/mg calibrated against GM-CSF reference standard (NIBSC cat no: 88/646). Reference use only. Specific activity is not routinely tested
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP04141
Molecular Mass 15 to 36 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse,pig

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).

Synonyms

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim

Publications

SpeciesTitle
human

Blood

GM-CSF-dependent pSTAT5 sensitivity is a feature with therapeutic potential in chronic myelomonocytic leukemia.

Authors - EricPadron
human

J Exp Med

Inherited TNFSF9 deficiency causes broad Epstein-Barr virus infection with EBV+ smooth muscle tumors.

Authors - Benjamin Fournier

Biomaterials

In situ repurposing of dendritic cells with CRISPR/Cas9-based nanomedicine to induce transplant tolerance.

Authors - Yue Zhang
human

Biosens Bioelectron

Accessing 3D microtissue metabolism: Lactate and oxygen monitoring in hepatocyte spheroids.

Authors - AndreasWeltin
human

J Innate Immun

Positive Feedback Loop of Long Noncoding RNA OASL-IT1 and Innate Immune Response Restricts the Replication of Zika Virus in Epithelial A549 Cells.

Authors - Yi Wang
human

J Biol Chem

Cysteine-rich domain 1 of CD40 mediates receptor self-assembly.

Authors - Cristian RSmulski