HumanKine® recombinant human GM-CSF protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity

0.08-0.8 ng/mL EC50

Species Reactivity

Human

Purity

>95%

Publications

14

Cat no : HZ-1002

Synonyms

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim

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Validation Data Gallery

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  The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

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  The activity was determined by the dose-dependent stimulation ofthe proliferation of human TF-1 cells (human erythroleukemic indicator cell line) using the Promega CellTiter96® Aqueous NonRadioactive Cell Proliferation Assay.

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Technical Specifications

• GeneID: 1437
• Species: Human
• Expression: HEK293
• Activity: 0.08-0.8 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P04141
• Molecular Mass: 15 to 36 kDa, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse,pig

Stability and Reconstitution

Stability and Storage	Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution	Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Whitepapers

Native Glycosylation Is Important For Biological Function and Activity of Recombinant Human Proteins

Time-Saving Dendritic Cell Generation No Medium Change - Less Reagents With HumanKine® GM-CSF and HumanKine IL4

Background

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).

Synonyms

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim

Publications

Species	Title
human	Am J Obstet Gynecol; Progesterone inhibits in vitro fetal membrane weakening.; Authors - Deepak Kumar
human	Int J Mol Sci; A Novel LRRK2 Variant p.G2294R in the WD40 Domain Identified in Familial Parkinson's Disease Affects LRRK2 Protein Levels.; Authors - Jun Ogata
human	J Immunol Methods; Assay system development to measure the concentration of sargramostim with high specificity in patients with autoimmune pulmonary alveolar proteinosis after single-dose inhalation.; Authors - Ryu Nakano
	Mol Ther Methods Clin Dev; A phase 1 study of a heterologous prime-boost vaccination involving a truncated HER2 sequence in patients with HER2-expressing breast cancer.; Authors - Sung-BaeKim
human	Inflamm Res; Activation of inflammatory cells and cytokines by peptide epitopes in vitro: a simple in-vitro screening assay for prioritizing them for in-vivo studies.; Authors - Lakshmi AMundkur
human	Placenta; Decidual GM-CSF is a critical common intermediate necessary for thrombin and TNF induced in-vitro fetal membrane weakening.; Authors - DKumar