HumanKine® recombinant human GM-CSF protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity

0.08-0.8 ng/mL EC50

Specific Activity

6.64x10^6 IU/mg calibrated against GM-CSF reference standard (NIBSC cat no: 88/646). Reference use only. Specific activity is not routinely tested

Species Reactivity

human,mouse,pig

Purity

>95%

Publications

15

Cat no : HZ-1002

Synonyms

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim

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Validation Data Gallery

• 

  The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

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  The activity was determined by dose-dependent stimulation of proliferation in human TF-1 cells (human erythroleukemic indicator cell line) measured using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

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Technical Specifications

• GeneID: 1437
• Species: Human
• Expression: HEK293
• Activity: 0.08-0.8 ng/mL EC50
• Specific Activity: 6.64x10^6 IU/mg calibrated against GM-CSF reference standard (NIBSC cat no: 88/646). Reference use only. Specific activity is not routinely tested
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P04141
• Molecular Mass: 15 to 36 kDa, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse,pig

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Whitepapers

Native Glycosylation Is Important For Biological Function and Activity of Recombinant Human Proteins

Time-Saving Dendritic Cell Generation No Medium Change - Less Reagents With HumanKine® GM-CSF and HumanKine IL4

Background

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).

Synonyms

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim

Publications

Species	Title
human	Am J Obstet Gynecol; Progesterone inhibits in vitro fetal membrane weakening.; Authors - Deepak Kumar
human	Int J Mol Sci; A Novel LRRK2 Variant p.G2294R in the WD40 Domain Identified in Familial Parkinson's Disease Affects LRRK2 Protein Levels.; Authors - Jun Ogata
human	J Immunol Methods; Assay system development to measure the concentration of sargramostim with high specificity in patients with autoimmune pulmonary alveolar proteinosis after single-dose inhalation.; Authors - Ryu Nakano
	Mol Ther Methods Clin Dev; A phase 1 study of a heterologous prime-boost vaccination involving a truncated HER2 sequence in patients with HER2-expressing breast cancer.; Authors - Sung-BaeKim
human	Inflamm Res; Activation of inflammatory cells and cytokines by peptide epitopes in vitro: a simple in-vitro screening assay for prioritizing them for in-vivo studies.; Authors - Lakshmi AMundkur
human	Placenta; Decidual GM-CSF is a critical common intermediate necessary for thrombin and TNF induced in-vitro fetal membrane weakening.; Authors - DKumar