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Animal-free Recombinant Human GM-CSF8 Publications

Catalog number: HZ-1002

HEK293 expressed Endotoxin-free Animal-component free

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Format: Lyophilized

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Technical Specifications

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GM-CSF activity assay

The activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) using the Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

GM-CSF purity

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue. R represents reducing conditions and NR represents non-reducing conditions.

GeneID:
1437
Species:
Human
Expression:
HEK293
Activity:
Typically ≤2 ng/mL EC50
Purity:
>95%
Molecular Mass:
15 to 36 kDa, monomer, glycosylated
Formulation
1x PBS, See Certificate of Analysis for details
Species Reactivity:
human,  mouse

Stability and Reconstitution

Stability and Storage:
Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.


Reconstitution:
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Whitepapers

Product Description

Product Description:

Animal-free Recombinant Human GM-CSF is produced in a human cell expression system in serum-free, chemically defined media. It has a demonstrated greater stability under cell culture conditions making it ideal for efficient generation of Human Dendritic Cells. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts, and T lymphocytes. Recombinant Human GM-CSF is a 15 to 36 kDa golbular protein consisting of 128 amino acids, containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites.



Background:

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).



Synonyms:
Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-&alpha CSF2, CSF 2, MGI1GM, MGI 1GM



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