- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human GM-CSF protein
Activity
0.08-0.8 ng/mL EC50
Specific Activity
6.64x10^6 IU/mg calibrated against GM-CSF reference standard (NIBSC cat no: 88/646). Reference use only. Specific activity is not routinely tested
Species Reactivity
human,mouse,pig
Purity
>95%
Cat no : HZ-1002
Synonyms
Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim
Validation Data Gallery
Technical Specifications
GeneID | 1437 |
Species | Human |
Expression | HEK293 |
Activity | 0.08-0.8 ng/mL EC50 |
Specific Activity | 6.64x10^6 IU/mg calibrated against GM-CSF reference standard (NIBSC cat no: 88/646). Reference use only. Specific activity is not routinely tested |
Purity | >95% |
Endotoxin | <1 EU/µg |
Accession Number | P04141 |
Molecular Mass | 15 to 36 kDa, monomer, glycosylated |
Formulation | 1x PBS, See Certificate of Analysis for details |
Species Reactivity | human,mouse,pig |
Stability and Reconstitution
Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
---|---|---|---|
Lyophilized | -20°C to -80°C | Until Expiry Date | |
Lyophilized | Room Temperature | 2 weeks | |
Reconstituted as per CofA | -20°C to -80°C | 6 months | |
Reconstituted as per CofA | 4°C | 1 week | |
Avoid repeated freeze-thaw cycles. |
Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA). |
Background
Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).
Synonyms
Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim
Publications
Species | Title |
---|---|
Int J Mol Sci A Novel LRRK2 Variant p.G2294R in the WD40 Domain Identified in Familial Parkinson's Disease Affects LRRK2 Protein Levels. | |
J Immunol Methods Assay system development to measure the concentration of sargramostim with high specificity in patients with autoimmune pulmonary alveolar proteinosis after single-dose inhalation. | |
Mol Ther Methods Clin Dev A phase 1 study of a heterologous prime-boost vaccination involving a truncated HER2 sequence in patients with HER2-expressing breast cancer. | |
Inflamm Res Activation of inflammatory cells and cytokines by peptide epitopes in vitro: a simple in-vitro screening assay for prioritizing them for in-vivo studies. | |
Placenta Decidual GM-CSF is a critical common intermediate necessary for thrombin and TNF induced in-vitro fetal membrane weakening. |