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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human GM-CSF protein

Activity

0.08-0.8 ng/mL

Species Reactivity

human

Purity

>95%

Cat no : HZ-1002

Synonyms

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim

Validation Data Gallery

  • The activity was determined by the dose-dependent stimulation ofthe proliferation of human TF-1 cells (human erythroleukemic indicator cell line) using the Promega CellTiter96® Aqueous NonRadioactive Cell Proliferation Assay.

    The activity was determined by the dose-dependent stimulation ofthe proliferation of human TF-1 cells (human erythroleukemic indicator cell line) using the Promega CellTiter96® Aqueous NonRadioactive Cell Proliferation Assay.

  • The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

    The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Technical Specifications

GeneID 1437
Species human
Expression HEK293
Activity 0.08-0.8 ng/mL
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP04141
Molecular Mass 15 to 36 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse,pig

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).

Synonyms

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim

Publications

SpeciesTitle
human

Am J Obstet Gynecol

Progesterone inhibits in vitro fetal membrane weakening.

Authors - Deepak Kumar
View Article
human

Int J Mol Sci

A Novel LRRK2 Variant p.G2294R in the WD40 Domain Identified in Familial Parkinson's Disease Affects LRRK2 Protein Levels.

Authors - Jun Ogata
View Article
human

J Immunol Methods

Assay system development to measure the concentration of sargramostim with high specificity in patients with autoimmune pulmonary alveolar proteinosis after single-dose inhalation.

Authors - Ryu Nakano
View Article

Mol Ther Methods Clin Dev

A phase 1 study of a heterologous prime-boost vaccination involving a truncated HER2 sequence in patients with HER2-expressing breast cancer.

Authors - Sung-BaeKim
View Article
human

Inflamm Res

Activation of inflammatory cells and cytokines by peptide epitopes in vitro: a simple in-vitro screening assay for prioritizing them for in-vivo studies.

Authors - Lakshmi AMundkur
View Article
human

Placenta

Decidual GM-CSF is a critical common intermediate necessary for thrombin and TNF induced in-vitro fetal membrane weakening.

Authors - DKumar
View Article