• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-10 protein

Activity

0.18-2.0 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1145

Synonyms

CSIF, IL 10, IL10, IL10A, interleukin 10, TGIF



Technical Specifications

GeneID 3586
Species Human
Expression HEK293
Activity 0.18-2.0 ng/mL EC50
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP22301
Molecular Mass 17 kDa, non-disulfide bonded homodimer, glycosylated
Formulation 10mM Tris-HCl pH 7.4 + 150mM NaCl, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

IL-10 is a non-covalent homodimeric glycoprotein with an apparent molecular mass of 17 kDa. IL-10 is produced by a variety of mammalian cell types including T cells, B cells, macrophages, and keratinocytes monocytes. It is an anti-inflammatory cytokine with multiple roles in inflammation and immunoregulation (PMID: 19161426; 9162098).

Synonyms

CSIF, IL 10, IL10, IL10A, interleukin 10, TGIF

Publications

SpeciesTitle
human

Stem Cell Res Ther

hPMSCs inhibit the expression of PD-1 in CD4+IL-10+ T cells and mitigate liver damage in a GVHD mouse model by regulating the crosstalk between Nrf2 and NF-κB signaling pathway.

Authors - Aiping Zhang