Validation Data Gallery
Technical Specifications
| GeneID | 3586 |
| Species | Human |
| Expression | HEK293 |
| Activity(Primary) | 0.18-2.0 ng/mL |
| Specific Activity | minimally 7.00 x 106 IU/mg |
| Purity | >95% |
| Endotoxin | <1 EU/μg |
| Accession Number | P22301 |
| Molecular Mass | 19 kDa reduced, 17 kDa non-reduced, non-disulfide bonded homodimer, glycosylated |
| Formulation | 10mM Tris-HCl pH 7.4 + 150mM NaCl, See Certificate of Analysis for details |
| Species Reactivity | human,mouse |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
Background
IL-10 is a non-covalent homodimeric glycoprotein with an apparent molecular mass of 17 kDa. IL-10 is produced by a variety of mammalian cell types including T cells, B cells, macrophages, and keratinocytes monocytes. It is an anti-inflammatory cytokine with multiple roles in inflammation and immunoregulation (PMID: 19161426; 9162098).
Synonyms
CSIF, IL 10, IL10, IL10A, interleukin 10, TGIF
Publications
| Species | Title |
|---|---|
Stem Cell Res Ther hPMSCs inhibit the expression of PD-1 in CD4+IL-10+ T cells and mitigate liver damage in a GVHD mouse model by regulating the crosstalk between Nrf2 and NF-κB signaling pathway. | |
Mol Med Tumor-associated macrophages display differential protein cargo sorting in extracellular vesicles associated with poor survival in ovarian cancer. | |
Cancer Lett Hypoxia-induced TREM1 promotes mesenchymal-like states of glioma stem cells via alternatively activating tumor-associated macrophages | |
Cancer Commun (Lond) CD24 is a promising immunotherapeutic target for enhancing efficacy of third-generation EGFR-TKIs on EGFR-mutated lung cancer. | |
Gut Microbes Microbiota-derived I3A protects the intestine against radiation injury by activating AhR/IL-10/Wnt signaling and enhancing the abundance of probiotics | |
bioRxiv Plasmacytoid dendritic cells in the intestine preferentially produce interferon lambda at homeostasis contributing to tonic localized innate immune responses. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Julie (Verified Customer) (10-23-2025) | Used in cell culture to polarise differentiated THP-1 cells to M2c phenotype. IL-10 was reconstituted and added to RPMI + Glutamax with 10% FBS and 1% PenStrep at a concentration of 20ng/mL with 1 x 10^6 cells/mL. Cells were then incubated for 48hrs with no media change, and CD163 (unconjugated + Coralite Plus 488 secondary, Proteintech) was used in flow cytometry to confirm polarisation.
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FH Farhat (Verified Customer) (03-08-2024) | We are using this cytokine to culture primary monocytes in vitro. The Humankine range of cytokines are amongst the best for cell culture of primary cells and we have always used them.
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