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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human PDGFaa protein

Activity

Typically ≤ 10 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1215

Synonyms

PDGF 1, PDGF A, PDGF subunit A, PDGF1, PDGFA, PDGFaa


  • The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

    The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

  • The activity was determined by the dose-dependent stimulation of the proliferation of 3T3 cells using the Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

    The activity was determined by the dose-dependent stimulation of the proliferation of 3T3 cells using the Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

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Technical Specifications

GeneID 5154
Species human
Expression HEK293
Activity Typically ≤ 10 ng/mL EC50
Purity >95%
Molecular Mass 35, 40 and 45 kDa, homodimer, glycosylated
Formulation 10 mM HOAc, See Certificate of Analysis for details
Species Reactivity human, mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 10 mM HOAc containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

PDGFaa is a member of the platelet-derived growth factor family. Four members of this family are mitogenic factors for cells of mesenchymal origin, and they are characterized by a motif of eight cysteines. PDGFaa plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, and chemotaxis. It is also required for a normal lung alveolar septum formation during embryogenesis, development of the gastrointestinal tract, and spermatogenesis. PDGFaa is also involved in oligodendrocyte development and l myelination process in the spinal cord and cerebellum PMID: 29408302; 29282077; 27002148).

Synonyms

PDGF 1, PDGF A, PDGF subunit A, PDGF1, PDGFA, PDGFaa

Publications

SpeciesTitle
human

Oxid Med Cell Longev

Autophagy Contributes to the Maintenance of Genomic Integrity by Reducing Oxidative Stress.

Authors - Wenqing Bu
mouse

Stem Cell Reports

Conversion of Fibroblast into Functional Leydig-like Cell Using Defined Small Molecules.

Authors - Yan Yang