HumanKine® recombinant human VEGF121 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

Typically ≤ 15 ng/mL EC50

Species Reactivity

human,pig,mouse

Purity

>95%

Publications

18

Cat no : HZ-1204

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGF121, VEGFA, VPF

---

Validation Data Gallery

• 

  Purity of recombinant human VEGF121 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

• 

  Recombinant human VEGF121 (HZ-1204) induces dose-dependent proliferation of the HUVEC (human umbilical vein endothelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HUVEC cells were treated with increasing concentrations of recombinant VEGF121 for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is less than 15 ng/mL.

---

Technical Specifications

• GeneID: 7422
• Species: Human
• Expression: HEK293
• Activity(Primary): Typically ≤ 15 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P15692
• Molecular Mass: 37 kDa, homodimer; 50 kDa, homotrimer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,pig,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulphide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells and is essential for physiological and pathological angiogenesis. Misregulations of this gene in mice result in abnormal embryonic blood vessel formation (PMID:10838264; 11329058).

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGF121, VEGFA, VPF

Publications

Species	Title
mouse,human	Circ Res; Esm1 modulates endothelial tip cell behavior and vascular permeability by enhancing VEGF bioavailability.; Authors - Susana FRocha
human	J Clin Invest; Sema3E-PlexinD1 signaling selectively suppresses disoriented angiogenesis in ischemic retinopathy in mice.; Authors - YokoFukushima
	Nat Commun; DutaFabs are engineered therapeutic Fab fragments that can bind two targets simultaneously.; Authors - Roland Beckmann
human	Elife; Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells.; Authors - NathanKumar
human	Oncogene; Progranulin and granulin-like protein as novel VEGF-independent angiogenic factors derived from human mesothelioma cells.; Authors - REguchi
human	Protein Cell; CRISPR/Cas9-mediated gene knockout reveals a guardian role of NF-κB/RelA in maintaining the homeostasis of human vascular cells.; Authors - Ping Wang