HumanKine® recombinant human VEGF121 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

1.5-9 ng/mL in Reporter cells, ≤ 15 ng/mL HUVEC cells

Species Reactivity

human,pig

Purity

>95%

Publications

19

Reviews (2)

Cat no : HZ-1204

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGF121, VEGFA, VPF

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Validation Data Gallery

• 

  Purity of recombinant human VEGF121 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

• 

  Recombinant human VEGF121 (HZ-1204) induces dose-dependent proliferation of the HUVEC (human umbilical vein endothelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HUVEC cells were treated with increasing concentrations of recombinant VEGF121 for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is less than 15 ng/mL.

• 

  Recombinant human VEGF121 (HZ-1204) induces dose-dependent luciferase production in a HEK293T VEGF luciferase reporter cell line. Luciferase production was assessed by the One-Step™ luciferase assay Kit. HEK293T VEGF luciferase reporter cells were treated with increasing concentrations of recombinant VEGF121 for 18 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 1.5-9 ng/mL

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Technical Specifications

• GeneID: 7422
• Species: Human
• Expression: HEK293
• Activity(Primary): 1.5-9 ng/mL in Reporter cells, ≤ 15 ng/mL HUVEC cells
• Activity(Secondary): 1.5-9 ng/mL
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P15692-9
• Molecular Mass: 17 and 21 kDa reduced, 28 to 40 kDa non-reduced, homodimer and homotrimer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,pig

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

• Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulphide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells and is essential for physiological and pathological angiogenesis. Misregulations of this gene in mice result in abnormal embryonic blood vessel formation (PMID:10838264; 11329058).

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGF121, VEGFA, VPF

Publications

Species	Title
mouse,human	Circ Res; Esm1 modulates endothelial tip cell behavior and vascular permeability by enhancing VEGF bioavailability.; Authors - Susana FRocha
human	J Clin Invest; Sema3E-PlexinD1 signaling selectively suppresses disoriented angiogenesis in ischemic retinopathy in mice.; Authors - YokoFukushima
	Nat Commun; DutaFabs are engineered therapeutic Fab fragments that can bind two targets simultaneously.; Authors - Roland Beckmann
human	Elife; Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells.; Authors - NathanKumar
human	Oncogene; Progranulin and granulin-like protein as novel VEGF-independent angiogenic factors derived from human mesothelioma cells.; Authors - REguchi
human	Protein Cell; CRISPR/Cas9-mediated gene knockout reveals a guardian role of NF-κB/RelA in maintaining the homeostasis of human vascular cells.; Authors - Ping Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christin (Verified Customer) (12-11-2024)

Great cytokine for generation of human iPSC-derived factories producing macrophage precursors (Haenseler protocol)

• Applications: Cell culture
• Cell Tissue Type: Human iPSC-derived macrophage precursors

FH

Harry (Verified Customer) (12-06-2024)

Used for differentiation of iPSC-derived endothelial cells. Cells expressed proper markers (CD31/PECAM-1). Works great

• Applications: Cell culture
• Cell Tissue Type: iPSC-derived endothelial cells and blood vessel spheroids