HumanKine® recombinant human VEGF121 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity

Typically ≤ 15 ng/mL EC50

Species Reactivity

human,pig

Purity

>95%

Publications

18

Cat no : HZ-1204

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGF121, VEGFA, VPF

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Validation Data Gallery

• 

  The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

• 

  The activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells) using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

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Technical Specifications

• GeneID: 7422
• Species: Human
• Expression: HEK293
• Activity: Typically ≤ 15 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P15692
• Molecular Mass: 37 kDa, homodimer; 50 kDa, homotrimer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,pig

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulphide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells and is essential for physiological and pathological angiogenesis. Misregulations of this gene in mice result in abnormal embryonic blood vessel formation (PMID:10838264; 11329058).

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGF121, VEGFA, VPF

Publications

Species	Title
human	Protein Cell; Modeling CADASIL vascular pathologies with patient-derived induced pluripotent stem cells.; Authors - Chen Ling
human	J Exp Clin Cancer Res; Retraction Note: Receptor tyrosine kinase inhibitor Sunitinib and integrin antagonist peptide HM-3 show similar lipid raft dependent biphasic regulation of tumor angiogenesis and metastasis.; Authors - Jialiang Hu
human	J Stem Cells Regen Med; Increased mesodermal and mesendodermal populations by BMP4 treatment facilitates human iPSC line differentiation into a cardiac lineage.; Authors - Maya Kimura
pig	NMR Biomed; MRI study of cryoinjury infarction in pig hearts: i. Effects of intrapericardial delivery of bFGF/VEGF embedded in alginate beads.; Authors - YYang
human	Int J Oncol; Calpain is involved in cisplatin-induced endothelial injury in an in vitro three-dimensional blood vessel model.; Authors - RyojiEguchi
human	Exp Cell Res; Recombinant human soluble thrombomodulin attenuates FK506-induced endothelial dysfunction through prevention of Akt inactivation.; Authors - Ryoji Eguchi