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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human VEGF121 protein

Activity

Typically ≤ 15 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1204

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGF121, VEGFA, VPF

Product data gallery View All

  • The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

    The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

  • The activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells) using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

    The activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells) using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

Technical Specifications

GeneID 7422
Species human
Expression HEK293
Activity Typically ≤ 15 ng/mL EC50
Purity >95%
Molecular Mass 37 kDa, homodimer; 50 kDa, homotrimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human, pig

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulphide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells and is essential for physiological and pathological angiogenesis. Misregulations of this gene in mice result in abnormal embryonic blood vessel formation (PMID:10838264; 11329058).

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGF121, VEGFA, VPF

Publications

SpeciesTitle
human

Protein Cell

Modeling CADASIL vascular pathologies with patient-derived induced pluripotent stem cells.

Authors - Chen Ling
View Article
human

J Exp Clin Cancer Res

Retraction Note: Receptor tyrosine kinase inhibitor Sunitinib and integrin antagonist peptide HM-3 show similar lipid raft dependent biphasic regulation of tumor angiogenesis and metastasis.

Authors - Jialiang Hu
View Article
human

J Stem Cells Regen Med

Increased mesodermal and mesendodermal populations by BMP4 treatment facilitates human iPSC line differentiation into a cardiac lineage.

Authors - Maya Kimura
View Article
pig

NMR Biomed

MRI study of cryoinjury infarction in pig hearts: i. Effects of intrapericardial delivery of bFGF/VEGF embedded in alginate beads.

Authors - YYang
View Article
human

Int J Oncol

Calpain is involved in cisplatin-induced endothelial injury in an in vitro three-dimensional blood vessel model.

Authors - RyojiEguchi
View Article
human

Exp Cell Res

Recombinant human soluble thrombomodulin attenuates FK506-induced endothelial dysfunction through prevention of Akt inactivation.

Authors - Ryoji Eguchi
View Article