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Animal-free Recombinant Human VEGF121
Humankine®13 Publications

Catalog number: HZ-1204

HEK293 expressed Endotoxin-free Animal-component free

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Format: Lyophilized

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Technical Specifications

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VEGF121 activity assay

The activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells) using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

VEGF121 purity

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue. R represents reducing conditions and NR represents non-reducing conditions.

GeneID:
7422
Species:
Human
Expression:
HEK293
Activity:
Typically ≤15 ng/mL EC50
Purity:
>95%
Molecular Mass:
37 kDa, homodimer; 50 kDa, homotrimer, glycosylated
Formulation
1x PBS, See Certificate of Analysis for details
Species Reactivity:
human, pig

Stability and Reconstitution

Stability and Storage:
Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.


Reconstitution:
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Product Description

Product Description:

Animal-free Recombinant Human VEGF121 is expressed in human 293 cells as a glycosylated cytokine with an apparent molecular mass of 37 kDa as dimer and 50 kDa as trimer. Production in human 293 cells offers authentic glycosylation, contributing to stability in cell growth media. The cytokine is produced in a human cell expression system with serum free, chemically defined media. VEGF121 is a potent growth and angioenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis amd vascular permeability.



Background:

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulphide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells and is essential for physiological and pathological angiogenesis. Misregulations of this gene in mice result in abnormal embryonic blood vessel formation (PMID:10838264; 11329058).



Synonyms:
Vascular Endothelial Growth Factor121, VPF Vascular Endothelial Growth Factor 121



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