SARS-CoV-2 S protein (944-1214 aa) Polyclonal antibody

SARS-CoV-2 S protein (944-1214 aa) Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Virus and More (4)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 28867-1-AP

Synonyms

S protein, SARS-CoV-2 Spike Glycoprotein (944-1214 aa), spike, spike protein



Tested Applications

Positive ELISA detected inRecombinant protein

Recommended dilution

ApplicationDilution
Enzyme-linked Immunosorbent Assay (ELISA)ELISA :
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

28867-1-AP targets SARS-CoV-2 S protein (944-1214 aa) in WB, IHC, IF, ELISA applications and shows reactivity with Virus samples.

Tested Reactivity Virus
Cited Reactivityhuman, mouse, monkey, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SARS-CoV-2 S protein (944-1214 aa) fusion protein Ag30685
Full Name SARS-CoV-2 Spike Protein
Calculated molecular weight 141 kDa
GenBank accession numberNC_045512
Gene symbol COVID-19 S Protein
Gene ID (NCBI) 43740568
RRIDAB_2881223
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). This antibody detects the spike protein of SARS and SARS-COV-2.

Publications

SpeciesApplicationTitle
monkey,hamsterIF

Nat Biomed Eng

High-throughput screening of genetic and cellular drivers of syncytium formation induced by the spike protein of SARS-CoV-2

Authors - Charles W F Chan
humanIF

Nat Immunol

Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules.

Authors - Matteo Stravalaci
IF

Cell Rep

Diverse immunoglobulin gene usage and convergent epitope targeting in neutralizing antibody responses to SARS-CoV-2.

Authors - Xiaojuan Zhou
IF

Pathogens

Hydrophobic Alpha-Helical Short Peptides in Overlapping Reading Frames of the Coronavirus Genome

Authors - Takashi Okura
mouseIHC,WB

Nat Commun

Essential role of TMPRSS2 in SARS-CoV-2 infection in murine airways

Authors - Naoko Iwata-Yoshikawa
humanIF

Int J Mol Sci

Modulation of NBAS-Related Functions in the Early Response to SARS-CoV-2 Infection

Authors - Valentina Granata