SARS-CoV-2 Spike Glycoprotein (428-506aa) Polyclonal antibody

SARS-CoV-2 Spike Glycoprotein (428-506aa) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

virus

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28901-1-AP

Synonyms



Tested Applications

Positive WB detected inRecombinant protein
Positive ELISA detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA :
Sample-dependent, check data in validation data gallery

Product Information

28901-1-AP targets SARS-CoV-2 Spike Glycoprotein (428-506aa) in WB, ELISA applications and shows reactivity with virus samples.

Tested Reactivity virus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SARS-CoV-2 Spike Glycoprotein (428-506aa) fusion protein Ag30684
Full Name SARS-CoV-2 Spike Protein
Calculated molecular weight 141 kDa
GenBank accession numberNC_045512
Gene symbol COVID-19 S Protein
Gene ID (NCBI) 43740568
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28901-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 428-506aa.

Protocols

Product Specific Protocols
WB protocol for SARS-CoV-2 Spike Glycoprotein (428-506aa) antibody 28901-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Irene (Verified Customer) (10-15-2020)

Tested the antibody for IF and western blot and it did not work for either application. There were minimal colocalization but also non-specific binding for IF.

  • Applications: Western Blot,Immunofluorescence