WB result of 4EBP1 antibody (60246-1-Ig, 1:4000) with si-Control and si-4EBP1 transfected K562 cells. This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.
K-562 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 60246-1-Ig(4EBP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.
