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CDK3 Polyclonal antibody

CDK3 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 55103-1-AP

Synonyms

CDK3, Cell division protein kinase 3, cyclin dependent kinase 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK3 Polyclonal antibody (55103-1-AP)

    WB analysis using 55103-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CDK3 Polyclonal antibody (55103-1-AP)

    WB analysis of MCF-7 using 55103-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CDK3 Polyclonal antibody (55103-1-AP)

    WB analysis of Jurkat using 55103-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CDK3 Polyclonal antibody (55103-1-AP)

    WB analysis of MCF-7 using 55103-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CDK3 Polyclonal antibody (55103-1-AP)

    IHC staining of human breast cancer using 55103-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55103-1-AP (CDK3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CDK3 Polyclonal antibody (55103-1-AP)

    IHC staining of human breast cancer using 55103-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55103-1-AP (CDK3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using CDK3 Polyclonal antibody (55103-1-AP)

    FC experiment of HeLa using 55103-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CDK3 (55103-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

Tested Applications

Positive WB detected inmouse brain tissue, MCF-7 cells, Jurkat cells, rat brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55103-1-AP targets CDK3 in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cyclin-dependent kinase 3
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberNM_001258
Gene Symbol CDK3
Gene ID (NCBI) 1018
RRIDAB_10804352
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDK3 belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. It is probably involved in the control of the cell cycle. CDK3 interacts with a yet unknown type of cyclin. It can phosphorylate histone H1. Endogenous Cyclin C/Cdk3 complexes are critical to promote exit from quiescence in an Rb-dependent manner. Cyclin C/Cdk3 complexes is a key regulators of cell cycle reentry in human cells. (PMID: 15130482 )The antibody is specific to CDK3.

Protocols

Product Specific Protocols
WB protocol for CDK3 antibody 55103-1-APDownload protocol
IHC protocol for CDK3 antibody 55103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Med

Identification of an EMT-related gene-based prognostic signature in osteosarcoma

Authors - Haoli Gong
View Article
mouseWB

Protein J

Characterization of Protein Profiling and mRNA Expression of LLC Exosomes.

Authors - Wen Zhang
View Article
humanWB

Cell Death Discov

Up-regulation of SLC27A2 suppresses the proliferation and invasion of renal cancer by down-regulating CDK3-mediated EMT.

Authors - Ning Xu
View Article