100 ul human peripheral blood were surface stained with 10 ul FITC Anti-Human CD14 (FITC-65056, Clone: UCHM-1), and 10 ul APC Anti-Human CD163 (APC-65169, Clone: GHI/61) or APC Mouse IgG1 isotype control. Samples were then treated with red blood cell lysis buffer and were gated for CD14+ monocytes for analysis of CD163 staining. Samples were not fixed.