Various lysates were subjected to SDS PAGE followed by western blot with 27156-1-AP (ATM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

WB result of ATM antibody (27156-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATM transfected HEK-293 cells.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-ATM (IP:27156-1-AP, 4ug; Detection:27156-1-AP 1:800) with HEK-293 cells lysate 1480 ug.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATM antibody (27156-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Various lysates were subjected to SDS PAGE followed by western blot with 67586-1-Ig (ATM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67586-1-Ig (ATM antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 67586-1-Ig (ATM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67586-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67586-1-Ig (ATM antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67586-1-PBS in a different storage buffer formulation.

ATM fusion protein Ag25920 SDS-PAGE

Various lysates were subjected to SDS PAGE followed by western blot with 11327-1-AP (ATRIP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

WB result of ATRIP antibody (11327-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATRIP transfected HeLa cells.

IP result of anti-ATRIP (IP:11327-1-AP, 4ug; Detection:11327-1-AP 1:5000) with HeLa cells lysate 1200 ug.