Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16541-1-AP (AXIN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16541-1-AP (AXIN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HT-1080 cells were subjected to SDS PAGE followed by western blot with 16541-1-AP (AXIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 68093-1-Ig (AXIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68093-1-Ig (AXIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68093-1-Ig (AXIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using AXIN1 antibody (68093-1-Ig, Clone: 1C4E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human AXIN1 (68093-1-Ig, Clone:1C4E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 68093-1-Ig (AXIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68093-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68093-1-Ig (AXIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68093-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68093-1-Ig (AXIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68093-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using AXIN1 antibody (68093-1-Ig, Clone: 1C4E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68093-1-PBS in a different storage buffer formulation.

1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human AXIN1 (68093-1-Ig, Clone:1C4E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68093-1-PBS in a different storage buffer formulation.

1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human AXIN1 (CL488-68093, Clone:1C4E8) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC1440 (AXIN1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using KHC1440 (AXIN1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using KHC1440 (AXIN1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using KHC1440 (AXIN1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using KHC1440 (AXIN1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using KHC1440 (AXIN1 IHC Kit).

AXIN1 fusion protein Ag10079 SDS-PAGE

AXIN1 fusion protein Ag9858 SDS-PAGE

Various lysates were subjected to SDS PAGE followed by western blot with 20540-1-AP (AXIN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20540-1-AP (AXIN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20540-1-AP (AXIN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).