"Alpha tubulin"

Various lysates were subjected to SDS PAGE followed by western blot with 11224-1-AP (alpha Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Lymphatic endothelial cells were stained for CD31 (green, Cat. No CL488-66065, 1:200) and alpha tubulin (magenta, Cat. 11224-1-AP, 1:200) with DAPI as a counterstain for visualizing the nucleus (yellow). CD31 is an endothelial cell adhesion protein and was conjugated to CoraLite-488 fluorescent dye to allow for direct visualization. Alpha tubulin is a cytoskeletal protein important in cell division and migration and was visualized with an Alexa Fluor 568 secondary antibody. Cells were fixed with 4% paraformaldehyde. Image credit: @Immunofluorescence on Instagram

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HEK-293 cells were stained with 0.2ug alpha Tubulin antibody (11224-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

Immunohistochemical analysis of paraffin-embedded human colon cancer using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:50 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded human colon cancer using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:50 (under 10x lens).

IP Result of anti-alpha Tubulin (IP:11224-1-AP, 3ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 5000ug.

Various lysates were subjected to SDS PAGE followed by western blot with 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP Result of anti-alpha Tubulin (IP:66031-1-Ig, 5ug; Detection:66031-1-Ig 1:1000) with HeLa cells lysate 2800ug.

1X10^6 HeLa cells were stained with 0.2ug alpha Tubulin antibody (66031-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IgG with dilution 1:100.

HeLa cells were subjected to SDS PAGE followed by western blot with 14555-1-AP (alpha Tubulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human testis using 14555-1-AP (alpha Tubulin antibody) at dilution of 1:50 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded human testis using 14555-1-AP (alpha Tubulin antibody) at dilution of 1:50 (under 10x lens).

Immunofluorescent analysis of HepG2 cells using 14555-1-AP (alpha Tubulin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Various lysates were subjected to SDS PAGE followed by western blot with 66200-1-Ig (acetylated Tubulin(Lys40) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunofluorescent images of MDCK cells stained for IFT88 rabbit pAb (13967-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 13967-1-AP, and Alexa Fluor 488-conjugated AffiniPure Goat anti-Mouse IgG (H+L) for 66200-1-Ig.

Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 66200-1-Ig (acetylated Tubulin(Lys40) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CL594-66200 (acetylated Tubulin(Lys40) antibody) at dilution of 1:100.