Various lysates were subjected to SDS PAGE followed by western blot with 11224-1-AP (alpha Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Lymphatic endothelial cells were stained for CD31 (green, Cat. No CL488-66065, 1:200) and alpha tubulin (magenta, Cat. 11224-1-AP, 1:200) with DAPI as a counterstain for visualizing the nucleus (yellow). CD31 is an endothelial cell adhesion protein and was conjugated to CoraLite-488 fluorescent dye to allow for direct visualization. Alpha tubulin is a cytoskeletal protein important in cell division and migration and was visualized with an Alexa Fluor 568 secondary antibody. Cells were fixed with 4% paraformaldehyde. Image credit: @Immunofluorescence on Instagram
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
1X10^6 HEK-293 cells were stained with 0.2ug alpha Tubulin antibody (11224-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:50 (under 10x lens).
IP Result of anti-alpha Tubulin (IP:11224-1-AP, 3ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 5000ug.