"Alpha tubulin"

Various lysates were subjected to SDS PAGE followed by western blot with 11224-1-AP (alpha Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Lymphatic endothelial cells were stained for CD31 (green, Cat. No CL488-66065, 1:200) and alpha tubulin (magenta, Cat. 11224-1-AP, 1:200) with DAPI as a counterstain for visualizing the nucleus (yellow). CD31 is an endothelial cell adhesion protein and was conjugated to CoraLite-488 fluorescent dye to allow for direct visualization. Alpha tubulin is a cytoskeletal protein important in cell division and migration and was visualized with an Alexa Fluor 568 secondary antibody. Cells were fixed with 4% paraformaldehyde. Image credit: @Immunofluorescence on Instagram

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HEK-293 cells were stained with 0.2ug alpha Tubulin antibody (11224-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

Immunohistochemical analysis of paraffin-embedded human colon cancer using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:50 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded human colon cancer using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:50 (under 10x lens).

IP Result of anti-alpha Tubulin (IP:11224-1-AP, 3ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 5000ug.

Various lysates were subjected to SDS PAGE followed by western blot with 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66031-1-Ig(alpha Tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66031-1-Ig (alpha Tubulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP Result of anti-alpha Tubulin (IP:66031-1-Ig, 5ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 2800ug.

1X10^6 HeLa cells were stained with 0.05 ug Anti-Human Alpha Tubulin (66031-1-Ig, Clone:1E4C11) and FITC-Goat anti-mouse IgG at dilution 1:100 (red), or stained with 0.05 ug isotype control and FITC-Goat anti-mouse IgG at dilution 1:100 (blue). Cells were fixed with 90% MeOH and permeabilized with 0.1% TritonX-100.

Western blot analysis of alpha-tubulin in various tissues and cell lines using Proteintech antibody HRP-60031 at a dilution of 1:5000.

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14555-1-AP (alpha Tubulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of HepG2 cells using 14555-1-AP (alpha Tubulin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14555-1-AP (Alpha Tubulin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite®488-conjugated version of this antibody, CL488-66031 (alpha Tubulin antibody), at dilution of 1:100.

Various lysates were subjected to SDS PAGE followed by western blot with CL488-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human Alpha Tubulin (CL488-66031, Clone:1E4C11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using Biotin-66031 (alpha Tubulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using Biotin-66031 (alpha Tubulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 Alpha Tubulin antibody (CL555-66031, Clone: 1E4C11 ) at dilution of 1:800.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Alpha Tubulin (CL555-66031, Clone:1E4C11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with CL594-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

1X10^6 HepG2 cells were stained with 0.20ug alpha Tubulin antibody (CL594-66031, red) and control antibody (blue). Fixed with 90% MeOH.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66031 (alpha Tubulin antibody) at dilution of 1:100.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite®594-conjugated version of this antibody, CL594-66031 (alpha Tubulin antibody), at dilution of 1:100.