HeLa cells were subjected to SDS PAGE followed by western blot with 10898-1-AP (PHF11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lymphoma using 10898-1-AP (PHF11 antibody) at dilution of 1:100 (under 10x lens).

Various lysates were subjected to SDS PAGE followed by western blot with 27903-1-AP (PIK3AP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 12295-1-AP (Prohibitin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12295-1-AP (Prohibitin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

WB result of Prohibitin 2 antibody (12295-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Prohibitin 2 transfected HeLa cells.

IP result of anti-Prohibitin 2 (IP:12295-1-AP, 3ug; Detection:12295-1-AP 1:1000) with HeLa cells lysate 1840ug.

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 40x lens).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Prohibitin 2 (12295-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 11200-1-AP (BAP31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11200-1-AP (BAP31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11200-1-AP (BAP31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BAP31 antibody (11200-1-AP) at dilution of 1:0 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), phalloidine stains F-actin (red).

IP result of anti-BAP31 (IP:11200-1-AP, 3ug; Detection:11200-1-AP 1:1000) with A431 cells lysate 3300ug.

1X10^6 HeLa cells were stained with 0.2ug BAP31 antibody (11200-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Various lysates were subjected to SDS PAGE followed by western blot with 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin was labelled in red with Phalloidin.

WB result of Prohibitin 2 antibody (66424-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Prohibitin 2 transfected HeLa cells.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug Cardinal Red™ Anti-Human BAP31 (CR-11200) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®488 BAP31 antibody (CL488-11200) at dilution of 1:200, CL594-Phalloidin (red).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human BAP31 (CL488-11200) (red) or 0.4 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL488-66424 (Prohibitin 2 antibody) at dilution of 1:100.