"BETA ACTIN"

Western blot analysis of beta-actin in various tissues and cell lines using Proteintech antibody 66009-1-Ig at a dilution of 1:20000. (Exposure time: 10 seconds).

Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using Beta Actin antibody (66009-1-Ig, Clone: 2D4H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 66009-1-Ig (beta Actin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

A549 cells (shcontrol and shRNA of Beta Actin) were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Mouse anti Pan-actin antibody) at dilution of 1:10000.

IP Result of anti-beta actin (IP:66009-1-Ig, 3ug; Detection:66009-1-Ig 1:10000) with HeLa cells lysate 3100ug.

1X10^6 HeLa cells were stained with 0.05 ug Anti-Human Beta Actin (66009-1-Ig, Clone:2D4H5) and FITC-Goat anti-mouse IgG at dilution 1:100 (red), or stained with 0.05 ug isotype control and FITC-Goat anti-mouse IgG at dilution 1:100 (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

Immunohistochemical analysis of paraffin-embedded human kidney using 66009-1-Ig at dilution of 1:500 (under 40x lens).

Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. beta actin is not expressed in adult heart thus we don't recommend it for heart tissue detection.

A549 cells (shcontrol and shRNA of Beta actin) were subjected to SDS PAGE followed by western blot with 20536-1-AP (ACTB antibody) at dilution of 1:500.

Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using Beta Actin antibody (20536-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20536-1-AP (beta actin Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20536-1-AP (beta actin Antibody) at dilution of 1:200 (under 40x lens).

1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Beta Actin (20536-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

Western blot analysis of beta actin in various tissues and cell lines using Proteintech antibody HRP-60008 at a dilution of 1:5000.

Various lysates were subjected to SDS PAGE followed by western blot with CL488-60008 (Beta Actin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-60008 (beta Actin antibody) at dilution of 1:200.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60008-1-Ig (beta Actin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Western blot analysis of Beta-actin in various tissues and cell lines using Proteintech antibody 60008-1-Ig at a dilution of 1:5000. Extra bands were detected in some species with unknown reason.

Immunohistochemical analysis of paraffin-embedded human colon using 60008-1-Ig(ACTB antibody) at dilution of 1:50 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human colon using 60008-1-Ig(ACTB antibody) at dilution of 1:50 (under 40x lens).

Western blot of Hela cell with anti-Actin-Beta (60008-1-Ig) at various dilutions.

arabidopsis whole plant tissue were subjected to SDS PAGE followed by western blot with 60008-1-Ig (beta actin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

1X10^6 HepG2 cells were stained with 0.2ug beta actin antibody (60008-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

Various lysates were subjected to SDS PAGE followed by western blot with HRP-66009 (beta Actin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using Biotin-60008 (beta Actin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using Biotin-60008 (beta Actin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).