Western blot analysis of beta-actin in various tissues and cell lines using Proteintech antibody 66009-1-Ig at a dilution of 1:20000. (Exposure time: 10 seconds).
Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using 66009-1-Ig (beta Actin antibody) at dilution of 1:1000 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Various lysates were subjected to SDS PAGE followed by western blot with 66009-1-Ig (beta Actin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
A549 cells (shcontrol and shRNA of Beta Actin) were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Mouse anti Pan-actin antibody) at dilution of 1:10000.
IP Result of anti-beta actin (IP:66009-1-Ig, 3ug; Detection:66009-1-Ig 1:10000) with HeLa cells lysate 3100ug.
1X10^6 HeLa cells were stained with 0.2ug beta actin antibody (66009-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IgG with dilution 1:100.
Immunohistochemical analysis of paraffin-embedded human kidney using 66009-1-Ig at dilution of 1:500 (under 40x lens).