Various lysates were subjected to SDS PAGE followed by western blot with 20899-1-AP (BRAF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 20899-1-AP (BRAF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 20899-1-AP (BRAF Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 20899-1-AP (BRAF Antibody) at dilution of 1:200 (under 40x lens).