Various lysates were subjected to SDS PAGE followed by western blot with 60178-1-Ig (BCL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60178-1-Ig (BCL2 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
WB result of BCL2 antibody (60178-1-Ig, 1:1000) with si-control and si-BCL2 transfected Jurkat cells.
IP Result of anti-BCL2 (IP:60178-1-Ig, 5ug; Detection:60178-1-Ig 1:1000) with U-937 cells lysate 4000ug.
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 60178-1-Ig (BCL2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
1X10^6 human peripheral blood lymphocytes were stained with 0.2 ug Anti-Human BCL2 (60178-1-Ig, Clone:4H8C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or 0.2 ug isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.
