Jurkat, Ramos, K-562 and Raw 264.7 cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of Hela cells, using BCL2L1 antibody 66020-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Bcl-XL (66020-1-Ig, Clone:4C12A6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.