Untreated and PNGase F-treated lysates of HT-29 cells and Caco-2 cells were subjected to SDS PAGE followed by western blot with 66666-1-Ig (CD133 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human CD133 (66666-1-Ig, Clone:2B8A2) (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Untreated and PNGase F-treated lysates of HT-29 cells and Caco-2 cells were subjected to SDS PAGE followed by western blot with 66666-1-Ig (CD133 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66666-1-Ig (CD133 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human CD133 (66666-1-Ig, Clone:2B8A2) (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66666-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 18470-1-AP (CD133 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18470-1-AP (CD133 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18470-1-AP (CD133 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CD133 antibody (18470-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human CD133 (18470-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 18495-1-AP (CD133 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

1X10^6 HT-29 cells were stained with 0.20 ug Anti-Human CD133 (18495-1-AP) (red) or 0.20 ug isotype control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Cells were fixed with 90% MeOH.

HT-29 cells were subjected to SDS PAGE followed by western blot with 19946-1-AP (CD133-1,2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

IP Result of anti-CD133-1,2,3 (IP:19946-1-AP, 4ug; Detection:19946-1-AP 1:300) with Y79 cells lysate 2000ug.

Y79 cells were subjected to SDS PAGE followed by western blot with 19945-1-AP (PROM1-1,2,3,5,7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

IP Result of anti-CD133-1,2,4,7 (IP:19945-1-AP, 4ug; Detection:19945-1-AP 1:300) with Y79 cells lysate 2000ug.

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19945-1-AP (CD133-1,2,4,7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19945-1-AP (CD133-1,2,4,7 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HT-29 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CD133 (CL488-66666, Clone:2B8A2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CoraLite®488 CD133 antibody (CL488-18470) at dilution of 1:200.