Jurkat cells (CD3+), MOLT-4 cells (CD3+), U-937 cells (CD3-), Raji cells (CD3-), mouse thymus tissue, and rat thymus tissue were subjected to SDS PAGE followed by western blot with 17617-1-AP (CD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB result of CD3 antibody (17617-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD3 transfected Jurkat cells.
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (17617-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD20 antibody (60271-1-Ig, Clone: 4A7G3, red).
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (17617-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD20 antibody (60271-1-Ig, Clone: 4A7G3, red).
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 Jurkat cells were stained with 0.5ug CD3 antibody (17617-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
IP result of anti-CD3 (IP:17617-1-AP, 4ug; Detection:17617-1-AP 1:1000) with Jurkat cells lysate 3600ug.