mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19068-1-AP (CD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP Result of anti-CD4 (IP:19068-1-AP, 4ug; Detection:19068-1-AP 1:500) with mouse thymus tissue lysate 2800ug.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD4 antibody (67786-1-Ig, Clone: 2H4A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) and 0.5 ug FITC Plus Anti-Mouse CD4 (FITC-65104, Clone: GK1.5) or 0.5 ug FITC Plus rat IgG2b isotype control. Cells were not fixed.

1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3 (17A2) (FITC-65077, Clone: 17A2) and 0.06 ug APC-Rat IgG2b isotype control (left) or 0.06 ug APC Anti-Mouse CD4 (APC-65104, Clone: GK1.5) (right). Cells were not fixed.

1X10^6 human peripheral blood lymphocytes were surface stained with 1 ug Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 1 ug isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD4 (65104-1-Ig, Clone:GK1.5) and FITC anti-rat IgG2b Antibody at dilution 1:100 (red), or 0.5 ug Control Antibody. Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and 5.00 ul CoraLite® Plus 488-conjugated Anti-Mouse CD4 (CL488-65141, Clone: RM4-5). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and CoraLite® Plus 488-conjugated Rat IgG2a isotype control antibody. Cells were not fixed.

Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD4 (CL488-65141, Clone: RM4-5) at dilution of 1:500.

1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) or CoraLite® Plus 647-Conjugated rat IgG2b isotype control. Cells were not fixed.

Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) at dilution of 1:500.