1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and 5.00 ul CoraLite® Plus 488-conjugated Anti-Mouse CD4 (CL488-65141, Clone: RM4-5). Cells were not fixed.
1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and CoraLite® Plus 488-conjugated Rat IgG2a isotype control antibody. Cells were not fixed.
Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD4 (CL488-65141, Clone: RM4-5) at dilution of 1:500.