1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) or 0.5 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2), FITC anti-rat IgG2b Antibody at dilution 1:100, and CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.