Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16485-1-AP (CDC25C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 K-562 cells were intracellularly stained with 0.2 ug Anti-Human CDC25C (16485-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

WB result of CDC25C antibody (16485-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDC25C transfected HeLa cells.

Various lysates were subjected to SDS PAGE followed by western blot with 16485-1-AP (CDC25C antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

IP Result of anti-CDC25C (IP:16485-1-AP, 4ug; Detection:16485-1-AP 1:1000) with K-562 cells lysate 3600ug.

rat brain tissue were subjected to SDS PAGE followed by western blot with 12958-1-AP (RASGRF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

human brain tissue were subjected to SDS PAGE followed by western blot with 12958-1-AP (RASGRF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

IP Result of anti-RASGRF1 (IP:12958-1-AP, 4ug; Detection:12958-1-AP 1:500) with rat brain tissue lysate 4000ug.

1X10^6 K-562 cells were intracellularly stained with 0.2 ug Anti-Human CDC25C (66912-1-Ig, Clone:2B6B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human CDC25C (CL488-66912, Clone:2B6B12) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67010-1-Ig (RASGRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

CDC25C fusion protein Ag10240 SDS-PAGE

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0805 (CDC25B IHC Kit).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC0805 (CDC25B IHC Kit).

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using KHC0805 (CDC25B IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using KHC0805 (CDC25B IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using KHC0805 (CDC25B IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using KHC0805 (CDC25B IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using KHC0805 (CDC25B IHC Kit).

HepG2 cells were subjected to SDS PAGE followed by western blot with 55031-1-AP (CDC25A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55031-1-AP (CDC25A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 55031-1-AP (CDC25A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IP Result of anti-CDC25A (IP:55031-1-AP, 4ug; Detection:55031-1-AP 1:300) with HeLa cells lysate 4000ug.