WB result of CDK1-Specific antibody (19532-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK1-Specific transfected HeLa cells.
Various lysates were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDK1-Specific (19532-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19532-1-AP (CDK1-Specific antibody at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19532-1-AP (CDK1-Specific antibody at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK1-Specific antibody (19532-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IP result of anti-CDK1-Specific (IP:19532-1-AP, 4ug; Detection:19532-1-AP 1:5000) with HeLa cells lysate 1600 ug.
IP result of anti-CDK1-Specific (IP:19532-1-AP, 4ug; Detection:19532-1-AP 1:5000) with HEK-293 cells lysate 1200 ug.