Non-treated PC-3 and MMS treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29012-1-AP (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:1000 incubated at room temperature for 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 CHEK2 antibody (CL488-13954) at dilution of 1:200.

Non-treated PC-3 and MMS treated PC-3 cells were subjected to SDS PAGE followed by western blot with 81740-1-RR (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

Non-treated HeLa and UV treated HeLa cells were subjected to SDS PAGE followed by western blot with 81740-1-RR (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

Non-treated PC-3 and MMS treated PC-3 cells were subjected to SDS PAGE followed by western blot with 81740-1-RR (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control. This data was developed using the same antibody clone with 81740-1-PBS in a different storage buffer formulation.

Non-treated HeLa and UV treated HeLa cells were subjected to SDS PAGE followed by western blot with 81740-1-RR (Phospho-CHEK2 (Thr68) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control. This data was developed using the same antibody clone with 81740-1-PBS in a different storage buffer formulation.

HL-60 cells were subjected to SDS PAGE followed by western blot with 13954-1-AP (Chk2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

HeLa cells were subjected to SDS PAGE followed by western blot with 13954-1-AP (Chk2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

IP result of anti-CHEK2 (IP:13954-1-AP, 4ug; Detection:13954-1-AP 1:4000) with HeLa cells lysate 1320 ug.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHEK2 antibody (13954-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phallodin (red).