Various lysates were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IP result of anti-CREB1 (IP:12208-1-AP, 4ug; Detection:12208-1-AP 1:2000) with HEK-293 cells lysate 1360 ug.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREB1 antibody (12208-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12208-1-AP (CREB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-Human CREB1 (12208-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).